Advances in Plant Ecophysiology Techniques

Description

This handbook covers the most commonly used techniques for measuring plant response to biotic and abiotic stressing factors, including: in vitro and in vivo bioassays; the study of root morphology, photosynthesis (pigment content, net photosynthesis, respiration, fluorescence and thermoluminiscence) and water status; thermal imaging; the measurement of oxidative stress markers; flow cytometry for measuring cell cycle and other physiological parameters; the use of microscope techniques for studying plant microtubules; programmed-cell-death; last-generation techniques (metabolomics, proteomics, SAR/QSAR); hybridization methods; isotope techniques for plant and soil studies; and the measurement of detoxification pathways, volatiles, soil microorganisms, and computational biology. Front Matter ....Pages i-xi In Vitro and In Vivo Bioassays (Mercedes Verdeguer)....Pages 1-13 Root Morphology (Antonio Lupini, Fabrizio Araniti, Antonio Mauceri, Maria Princi, Antonino Di Iorio, Agostino Sorgonà et al.)....Pages 15-28 Plant Photosynthetic Pigments: Methods and Tricks for Correct Quantification and Identification (Beatriz Fernández-Marín, José Ignacio García-Plazaola, Antonio Hernández, Raquel Esteban)....Pages 29-50 Measuring Photosynthesis and Respiration with Infrared Gas Analysers (Cyril Douthe, Jorge Gago, Miquel Ribas-Carbó, Rubén Núñez, Nuria Pedrol, Jaume Flexas)....Pages 51-75 Chlorophyll Fluorescence: A Practical Approach to Study Ecophysiology of Green Plants (Amarendra Narayan Mishra)....Pages 77-97 Thermoluminescence: A Tool to Study Ecophysiology of Green Plants (Amarendra Narayan Mishra)....Pages 99-108 Determining Plant Water Relations (Gorka Erice, María Luisa Pérez-Bueno, Mónica Pineda, Matilde Barón, Ricardo Aroca, Mónica Calvo-Polanco)....Pages 109-134 Thermal Imaging and Infrared Sensing in Plant Ecophysiology (Hamlyn G. Jones)....Pages 135-151 Photoprotection and Photo-Oxidative Stress Markers As Useful Tools to Unravel Plant Invasion Success (Erola Fenollosa, Sergi Munné-Bosch)....Pages 153-175 Reactive Oxygen Species and Antioxidant Enzymatic Systems in Plants: Role and Methods (Teresa Papalia, Maria Rosaria Panuccio, Maria Sidari, Adele Muscolo)....Pages 177-193 Flow Cytometric Measurement of Different Physiological Parameters (Fabrizio Araniti, Teodoro Coba de la Peña, Adela M. Sánchez-Moreiras)....Pages 195-213 Flow Cytometry: Cell Cycle (Teodoro Coba de la Peña, Adela M. Sánchez-Moreiras)....Pages 215-229 Mitotic Index (Elisa Graña)....Pages 231-240 Fluorescent Probes and Live Imaging of Plant Cells (Elfrieda Fodor, Ferhan Ayaydin)....Pages 241-251 Confocal and Transmission Electron Microscopy for Plant Studies (Adela M. Sánchez-Moreiras, Marianna Pacenza, Fabrizio Araniti, Leonardo Bruno)....Pages 253-271 Plant Programmed Cell Death (PCD): Using Cell Morphology as a Tool to Investigate Plant PCD (T. John Conway, Paul F. McCabe)....Pages 273-281 Visualization of Plant Microtubules (Elisa Graña)....Pages 283-294 Multiprobe In-Situ Hybridization to Whole Mount Arabidopsis Seedlings (Leonardo Bruno, Fabrizio Araniti, Olimpia Gagliardi)....Pages 295-308 Proteomics Analysis of Plant Tissues Based on Two-Dimensional Gel Electrophoresis (Jesus V. Jorrín-Novo, Luis Valledor-González, Mari A. Castillejo-Sánchez, Rosa Sánchez-Lucas, Isabel M. Gómez-Gálvez, Cristina López-Hidalgo et al.)....Pages 309-322 Metabolomics and Metabolic Profiling: Investigation of Dynamic Plant-Environment Interactions at the Functional Level (Dominik Skoneczny, Paul A. Weston, Leslie A. Weston)....Pages 323-345 SAR/QSAR (Marta Teijeira, María Celeiro)....Pages 347-361 Elucidating the Phytotoxic Potential of Natural Compounds (Adela M. Sánchez-Moreiras, Elisa Graña, Carla Díaz-Tielas, David López-González, Fabrizio Araniti, María Celeiro et al.)....Pages 363-378 Exploring Plants Strategies for Allelochemical Detoxification (Margot Schulz, Meike Siebers, Nico Anders)....Pages 379-399 Chemical Characterization of Volatile Organic Compounds (VOCs) Through Headspace Solid Phase Micro Extraction (SPME) (Fabrizio Araniti, Sebastiano Pantò, Antonio Lupini, Francesco Sunseri, Maria Rosa Abenavoli)....Pages 401-417 Carbon Radiochemicals (14C) and Stable Isotopes (13C): Crucial Tools to Study Plant-Soil Interactions in Ecosystems (Geneviève Chiapusio, Dorine Desalme, Philippe Binet, François Pellissier)....Pages 419-437 Stable-Isotope Techniques to Investigate Sources of Plant Water (Adrià Barbeta, Jérôme Ogée, Josep Peñuelas)....Pages 439-456 Soil Microorganisms (Joana Costa, Rui S. Oliveira, Igor Tiago, Ying Ma, Cristina Galhano, Helena Freitas et al.)....Pages 457-482 Computational Approach to Study Ecophysiology (Bibhuti Prasad Barik, Amarendra Narayan Mishra)....Pages 483-497