ENGLISH

Light Microscopy: Methods and Protocols

By Hélio Chiarini-Garcia , Gleydes Gambogi Parreira , Fernanda R.C.L. Almeida (auth.) , Rossana C. N. Melo (eds.)

Book information

Publisher
Humana Press
Year
2011
ISBN
1607619490, 9781607619499, 9781607619505
DOI
10.1007/978-1-60761-950-5
Open Library ID
OL25176856M
Language
english
Format
PDF
Filesize
10 MB (10866881 bytes)
Series
Methods in Molecular Biology 689
Edition
1
Pages
204\249
Topic
Biology Molecular
Time added
2011-06-04 13:46:07
Cell Biology

Description

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.

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