ENGLISH

Gapmers: Methods and Protocols

By Toshifumi Yokota , Rika Maruyama

Book information

Publisher
Springer US;Humana
Year
2020
ISBN
9781071607701, 9781071607718
DOI
10.1007/978-1-0716-0771-8
Language
english
Format
PDF
Filesize
6 MB (6318992 bytes)
Series
Methods in Molecular Biology 2176
Edition
1st ed.
Pages
XI, 247\246
Time added
2021-01-06 05:41:07
Biomedicine; Human Genetics

Description

This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gapmers: Methods and Protocols aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier.

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