ENGLISH

SV40 Protocols (Methods in Molecular Biology Vol 165)

By Leda Raptis

Book information

Year
2001
ISBN
9780896036536, 9781592591176, 0896036537
Open Library ID
OL8253666M
Language
english
Format
PDF
Filesize
2 MB (2619328 bytes)
Edition
1st
Pages
310\324
Topic
Biology Molecular
Time added
2011-06-04 13:46:07

Description

A panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and the use of transgenic mouse models based on the SV40 large T antigen. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.

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