Functional Proteomics and Nanotechnology-Based Microarrays

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This volume introduces in a coherent and comprehensive fashion the Pan Stanford Series on Nanobiobiotechnology by defining and reviewing the major sectors of Nanobiotechnology and Nanobiosciences with respect to the most recent developments. Nanobiotechnology indeed appears capable of yielding a scientific and industrial revolution along the routes correctly foreseen by the numerous programs on Nanotechnology launched over the last decade by numerous Councils and Governments worldwide, beginning in the late 1995 by the Science and Technology Council in Italy and by the President Clinton in USA and ending this year with President Putin in Russian Federation.  Read more... Abstract: This volume introduces in a coherent and comprehensive fashion the Pan Stanford Series on Nanobiobiotechnology by defining and reviewing the major sectors of Nanobiotechnology and Nanobiosciences with respect to the most recent developments. Nanobiotechnology indeed appears capable of yielding a scientific and industrial revolution along the routes correctly foreseen by the numerous programs on Nanotechnology launched over the last decade by numerous Councils and Governments worldwide, beginning in the late 1995 by the Science and Technology Council in Italy and by the President Clinton in USA and ending this year with President Putin in Russian Federation Content: Cover Half Title Title Page Copyright Page Dedication Table of Contents Preface 1: NANOTECHNOLOGY APPLICATIONS OF NUCLEIC ACID PROGRAMMABLE PROTEIN ARRAYS 1.1 Introduction 1.2 Materials and Methods 1.2.1 The FLEXGene repository 1.2.2 Nucleic Acid-Programmable Protein Array (NAPPA) 1.2.3 Protein-protein interactions 1.3 Results 1.3.1 Atomic force microscopy (AFM) 1.3.2 Nanogravimetry 1.3.3 Mass spectrometry (MS) 1.3.4 Anodic porous alumina (APA) 1.3.5 Fluorescence via DNASER and bioinformatics 1.3.6 Transcription translation kit 1.4 Discussion 1.5 Conclusions AcknowledgmentsReferences 2: BIOINFORMATICS AND FLUORESCENCE DNASER FOR NAPPA STUDIES ON CELL TRANSFORMATION AND CELL CYCLE 2.1 Introduction 2.2 Materials and Methods 2.2.1 The experimental layout 2.2.2 Procedure of NAPPA expression and DNASER analysis 2.3 Results 2.3.1 NAPPA DNASER imaging 2.3.2 NAPPA-targeted prediction of gene interactions relevant to progressing between phases of cell cycle of human T lymphocytes 2.3.3 NAPPA-targeted prediction of protein interactions relevant to differences between lymphoma and normal T cells 2.3.4 Planned experimentation 2.4 Conclusions AcknowledgmentsReferences 3: LABEL FREE DETECTION OF NAPPA VIA MASS SPECTROMETRY 3.1 Introduction 3.2 Materials and Methods 3.2.1 The experimental layout 3.2.2 Fabrication of NAPPA 3.2.3 NAPPA expression 3.2.4 Autoflex analysis 3.3 Results 3.3.1 Human kinase NAPPA 3.3.2 NAPPA for MS 3.3.2.1 Matching algorithm 3.4 Conclusions Acknowledgments References 4: LABEL FREE NAPPA VIA NANOGRAVIMETRY 4.1 Introduction 4.2 Materials and Methods 4.2.1 QCM-frequency technique 4.2.2 QCM-D dissipation (quality) factor technique 4.3 Results 4.4 Conclusions and Suggestions Acknowledgments 6.2 Materials and Methods6.2.1 Technical details for NAPPA imaging by AFM 6.3 Results 6.3.1 NAPPA investigations with the functionalization of cantilever and the measurement of interaction forces 6.4 Conclusions and Advancements Acknowledgments References 7: CELL FREE EXPRESSION AND APA FOR NAPPA AND PROTEIN NANOCRYSTALLOGRAPHY 7.1 Introduction 7.2 APA and NAPPA Microarray 7.3 Background of Cell-Free Protein Synthesis 7.4 APA-Cell Free and Protein Nanocrystallography 7.5 Materials and Methods 7.5.1 The PURExpress system and its advantages 7.5.2 APA template preparation