ENGLISH

Recombinant Glycoprotein Production

Book information

Publisher
Humana Pr Inc, Picanȯ-castro, Virgn̕ia, Swiech, Kamilla
Year
2017
ISBN
978-1-4939-7312-5, 1493973126, 978-1-4939-7311-8
Language
english
Format
PDF
Filesize
6 MB (6641977 bytes)
Pages
\282
Time added
2017-10-15 16:00:00

Description

This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field Front Matter ....Pages i-xi Platforms for Recombinant Therapeutic Glycoprotein Production (Amanda Mizukami, Angelo Luis Caron, Virgínia Picanço-Castro, Kamilla Swiech)....Pages 1-14 Uncovering Innovation Features and Emerging Technologies in Molecular Biology through Patent Analysis (Cristiano Gonçalves Pereira, Geciane Silveira Porto)....Pages 15-34 Front Matter ....Pages 35-35 Production of Full-Length Antibody by Pichia pastoris (Adam Nylen, Ming-Tang Chen)....Pages 37-48 Human Cells as Platform to Produce Gamma-Carboxylated Proteins (Aline de Sousa Bomfim, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, Elisa Maria de Sousa Russo)....Pages 49-61 Production of Recombinant Factor VIII in Human Cell Lines (Daianne Maciely Carvalho Fantacini, Virgínia Picanço-Castro)....Pages 63-74 Strategies to Suspension Serum-Free Adaptation of Mammalian Cell Lines for Recombinant Glycoprotein Production (Angelo Luis Caron, Rafael Tagé Biaggio, Kamilla Swiech)....Pages 75-85 Production of Recombinant Rabies Virus Glycoprotein by Insect Cells in a Single-Use Fixed-Bed Bioreactor (Daniella C. Ventini-Monteiro, Renato M. Astray, Carlos A. Pereira)....Pages 87-94 Cell-Free Production of Protein Biologics Within 24 H (Challise J. Sullivan, Erik D. Pendleton, John Dresios)....Pages 95-107 Demonstration-Scale High-Cell-Density Fermentation of Pichia pastoris (Wan-Cang Liu, Ping Zhu)....Pages 109-116 Large-Scale Transient Transfection of Suspension Mammalian Cells for VLP Production (Laura Cervera, Amine A. Kamen)....Pages 117-127 Bioreactor-Based Production of Glycoproteins in Plant Cell Suspension Cultures (Tanja Holland, Johannes Felix Buyel)....Pages 129-146 Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production (Yuzhou Fan, Daniel Ley, Mikael Rørdam Andersen)....Pages 147-161 Strategies to Develop Therapeutic N- and O-Hyperglycosylated Proteins (Agustina Gugliotta, Natalia Ceaglio, Marina Etcheverrigaray, Ricardo Kratje, Marcos Oggero)....Pages 163-181 Expression of Glycosylated Proteins in Bacterial System and Purification by Affinity Chromatography (Carlos Alexandre Breyer, Marcos Antonio de Oliveira, Adalberto Pessoa Jr)....Pages 183-191 Front Matter ....Pages 193-193 Purification Methods for Recombinant Factor VIII Expressed in Human Liver SK-Hep Cells (Vladimir Granovski, Mario Soares Abreu-Neto, Dimas Tadeu Covas)....Pages 195-202 Purification Method for Recombinant hG-CSF by Affinity Chromatography (Bruna Samham Archangelo, Elisa Maria de Sousa Russo)....Pages 203-209 Microplate-Based Method for High-Throughput Screening (HTS) of Chromatographic Conditions Studies for Recombinant Protein Purification (Rimenys J. Carvalho, Thayana A. Cruz)....Pages 211-220 Purification and Autoactivation Method for Recombinant Coagulation Factor VII (Vladimir Granovski, Marcela C. C. Freitas, Mario Soares Abreu-Neto, Dimas T. Covas)....Pages 221-226 Front Matter ....Pages 227-227 Preparation of Immunoliposomes by Direct Coupling of Antibodies Based on a Thioether Bond (Raquel Petrilli, Josimar O. Eloy, Robert J. Lee, Renata F. V. Lopez)....Pages 229-237 Polyester-Based Nanoparticles for the Encapsulation of Monoclonal Antibodies (Flávia Sousa, Pedro Fonte, Andreia Cruz, Patrick J. Kennedy, Inês Mendes Pinto, Bruno Sarmento)....Pages 239-253 Polyester-Based Nanoparticles for Delivery of Therapeutic Proteins (Pedro Fonte, Flávia Sousa, Bruno Sarmento)....Pages 255-274 Quantification of Coagulation Factor VIII by Selective Reaction Monitoring (Edson Galdino do Nascimento Filho, Guilherme Pauperio Lanfredi, Mário Soares Abreu-Neto, Dimas Tadeu Covas, Vitor Marcel Faça)....Pages 275-282 Back Matter ....Pages 283-285

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