In Vitro Differentiation of T-Cells: Methods and Protocols

Description

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals. Front Matter ....Pages i-xi Flow Cytometry Analysis to Identify Human CD8+ T Cells (Jacqueline Flynn, Paul Gorry)....Pages 1-13 Flow Cytometry Analysis to Identify Human CD4+ T Cell Subsets (Jacqueline Flynn, Paul Gorry)....Pages 15-25 Gene Modification and Immunological Analyses for the Development of Immunotherapy Utilizing T Cells Redirected with Antigen-Specific Receptors (Toshiki Ochi, Masaki Maruta, Naoto Hirano)....Pages 27-39 In Vitro Conversion of Activated T Cells into Stem Cell Memory-Like T Cells (Taisuke Kondo, Yuki Imura, Makoto Ando, Shunsuke Chikuma, Akihiko Yoshimura)....Pages 41-51 Human iPSC Generation from Antigen-Specific T Cells (Toshinobu Nishimura, Yukiko Murmann, Hiromitsu Nakauchi)....Pages 53-57 In Vitro Differentiation of T Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells (Shoichi Iriguchi, Shin Kaneko)....Pages 59-70 Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC (Yohei Kawai, Shin Kaneko)....Pages 71-75 In Vitro Differentiation of T Cell: From Human iPS Cells in Feeder-Free Condition (Yutaka Yasui, Yasumichi Hitoshi, Shin Kaneko)....Pages 77-80 Differentiating CD8αβ T Cells from TCR-Transduced iPSCs for Cancer Immunotherapy (Atsutaka Minagawa, Shin Kaneko)....Pages 81-84 In Vitro Differentiation of T Cell: From CAR-Modified T-iPSC (Tatsuki Ueda, Shin Kaneko)....Pages 85-91 In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells (Shoichi Iriguchi, Shin Kaneko)....Pages 93-106 An Improved Method to Produce Clinical-Scale Natural Killer Cells from Human Pluripotent Stem Cells (Huang Zhu, Dan S. Kaufman)....Pages 107-119 In Vitro Detection of Cellular Adjuvant Properties of Human Invariant Natural Killer T Cells (Rong Zhang, Shuichi Kitayama, Tianyi Liu, Norihiro Ueda, Yumi Tokumitsu, Hiroaki Mashima et al.)....Pages 121-130 In Vitro Differentiation of T Cells from Murine Pluripotent Stem Cells (Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song)....Pages 131-141 Clonogenic Culture of Mouse Thymic Epithelial Cells (Miho Sekai, Jianwei Wang, Yoko Hamazaki)....Pages 143-153 Single-Cell Transcriptome Analysis of T Cells (Willem Van Der Byl, Simone Rizzetto, Jerome Samir, Curtis Cai, Auda A. Eltahla, Fabio Luciani)....Pages 155-205 Structural Modeling of Lymphocyte Receptors and Their Antigens (Songling Li, Jan Wilamowski, Shunsuke Teraguchi, Floris J. van Eerden, John Rozewicki, Ana Davila et al.)....Pages 207-229 Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers (Tinhinane Fali, Camille K’Ros, Victor Appay, Delphine Sauce)....Pages 231-243 Generation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells (Hideyuki Oguro)....Pages 245-257 Using the Inducible Caspase-9 Suicide-Safeguard System with iPSC and Bioluminescent Tracking (Jonathan Villanueva, Toshinobu Nishimura, Hiromitsu Nakauchi)....Pages 259-264 Back Matter ....Pages 265-267