ENGLISH

Two-Dimensional Electrophoresis Protocols

By Richard Smith (auth.) , Raymond Tyther , David Sheehan (eds.)

Book information

Publisher
Humana Press
Year
2009
ISBN
9781588299376, 1588299376
DOI
10.1007/978-1-59745-281-6
LCC
QP519.9.E434 T86 2009
Open Library ID
OL24878954M
Language
english
Format
PDF
Filesize
6 MB (6342965 bytes)
Series
Methods in molecular biology 519
Edition
1
Pages
546\538
Topic
Biology Genetics
Library
kolxoz-dop
Time added
2010-02-18 13:16:04
Protein Science; Proteomics; Electrophoresis

Description

Two-dimensional electrophoresis (2-DE) remains one of the most popular techniques for proteomic comparisons. Revolutionized by the development of immobilized pH gradient strips, this vital process has benefited greatly from recent developments in staining, mass spectrometry, and bioinformatics. In Two-Dimensional Electrophoresis Protocols, expert researchers examine these cutting edge proteomics methodologies, explore their application to a wide range of biological materials, and look at how these developments have helped standardize the design, execution, and analysis of proteomics experiments. Chapters reflect key steps in 2-DE experiments, including sample preparation, staining, post-translational modification, spot identification, and bioinformatics. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and reader-friendly, Two-Dimensional Electrophoresis Protocols encourages newcomers to apply powerful 2-DE techniques to their own research, while also providing current and essential information for seasoned scientists.

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2010 · PDF