Molecular Biological and Immunological Techniques and Applications for Food Chemists

Description

A guide to using molecular biology and immunological methods for the analysis of food Many of the analytical problems that food chemists face in the lab cannot be solved by chemistry alone, and so analytical chemists are turning to molecular biology and immunology for alternative approaches. Molecular Biological and Immunological Techniques and Applications for Food Chemists comprehensively explains the most important molecular biology and immunology methods, and illustrates their application in food analysis. Written by a distinguished group of experts, the coverage includes: Molecular Biological Methods—techniques explained, laboratory layout, PCR, real-time PCR, RFLP, SSCP, and sequencing Molecular Biology Applications—meat, genetically modified organisms (GMOs), food allergens, offal, and fish Immunological Methods—techniques explained and antibody-based detection methods Immunology Applications—animal speciation, international food allergen regulations (except Japanese), Japanese regulations and buckwheat allergen detection, egg allergen detection, soy allergen detection, milk allergen detection, gluten allergen detection, nut allergen detection, fish allergen detection, lupin allergen detection, mustard allergen detection, and celery allergen detection Clearly written and consistently edited to provide information to a wide range of readers, Molecular Biological and Immunological Techniques and Applications for Food Chemists offers an up-to-date reference for food scientists in government and industry, policymakers, and graduate-level students of food science, technology, and engineering. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file.Content: Chapter 1 Molecular Biology Laboratory Layout (pages 1–39): Rainer SchubbertChapter 2 Polymerase Chain Reaction (pages 41–58): Hermann BrollChapter 3 Quantitative Real?Time PCR (pages 59–83): Hermann BrollChapter 4 Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis (pages 85–103): Klaus Pietsch and Hans?Ulrich WaiblingerChapter 5 Single?Stranded Conformation Polymorphism Analysis (pages 105–117): Hartmut RehbeinChapter 6 Sequencing (pages 119–132): Rainer SchubbertChapter 7 Meat (pages 133–155): Ines LaubeChapter 8 Genetically Modified Organisms (pages 157–174): Bert PoppingChapter 9 Detection of Food Allergens (pages 175–198): Carmen Diaz?Amigo and Bert PoppingChapter 10 Offal (pages 199–208): Neil HarrisChapter 11 Aquatic Food (pages 209–219): Hartmut RehbeinChapter 12 Antibody?Based Detection Methods: From Theory to Practice (pages 221–245): Carmen Diaz?AmigoChapter 13 Animal Specification in Speciation (pages 247–266): Bruce W. Ritter and Laura AllredChapter 14 International Regulatory Environment for Food Allergen Labeling (pages 267–292): Samuel Benrejeb Godefroy and Bert PoppingChapter 15 Japanese Regulations and Buckwheat Allergen Detection (pages 293–309): Hiroshi Akiyama, Shinobu Sakai, Reiko Adachi and Reiko TeshimaChapter 16 Egg Allergen Detection (pages 311–334): Masahiro ShojiChapter 17 Soy Allergen Detection (pages 335–348): Marcello Gatti and Cristina FerrettiChapter 18 Milk Allergen Detection (pages 349–357): Sabine BaumgartnerChapter 19 Gluten Detection (pages 359–376): Ulrike Immer and Sigrid Haas?LauterbachChapter 20 Nut Allergen Detection (pages 377–406): Richard Fielder, Warren Higgs and Katie BardenChapter 21 Fish Allergen Detection (pages 407–422): Christiane Kruse F?steChapter 22 Lupin Allergen Detection (pages 423–444): Christiane Kruse F?steChapter 23 Mustard Allergen Detection (pages 445–450): Anne E. Ryan and Michael S. RyanChapter 24 Celery Allergen Detection (pages 451–457): Charlotta Engdahl Axelsson