Electrophoresis Fundamentals: Essential Theory and Practice

Description

The electrophoresis techniques are used in medicine, biochemistry, analytical chemistry, and biology to separate soluble and insoluble proteins, nucleic acids, chromosomes, viruses, as well as lysosomes, mitochondria, ribosomes and other cell organelles, red cells, tissue cells, and parasites. This book provides a view over the old electrophoresis techniques, as well as the recent developments in electrophoresis. Electrophoresis Fundamentals is based on the recent book Electrophoresis: Theory and Practice published in 2020 by De Gruyter. The previous book combines theory and technical applications with troubleshooting and problem solving. While Electrophoresis is intended for specialists, Electrophoresis Fundamentals is a book for laboratory technicians, students, biochemists, general practitioners, and more. Discusses modern electrophoresis methods such as the PCR technique, which is used to detect COVID-19. Contains troubleshooting and problem solving. Intended for students, chemists, biochemists, geneticists, physicians, and more. Preface About the Author Contents Abbreviations 1 Fundamentals of electrophoresis 1.1 Electric double layer of a charged particle 1.2 Proteins and nucleic acids form polyions in solution 1.3 Electrophoresis is running in buffers 1.4 Polyions are moving in electric field 1.5 Electrophoresis is carried out in different solid media 1.6 General theory of electrophoresis 1.7 Electrophoresis instrumentation 1.8 Classification of electrophoretic methods 2 Electrophoresis of proteins 2.1 Cellulose acetate electrophoresis of proteins 2.2 Agarose gel electrophoresis of proteins 2.3 Immunoelectrophoresis 2.4 Affinity electrophoresis 2.5 Polyacrylamide gel zone electrophoresis of proteins 2.6 Isotachophoresis of proteins 2.7 Disc-electrophoresis of proteins 2.8 Isoelectric focusing of proteins 2.9 Free-flow electrophoresis of proteins 2.10 Capillary electrophoresis of proteins 2.11 Two-dimensional electrophoresis 2.12 Preparative electrophoresis of proteins 2.13 Microchip electrophoresis of proteins 2.14 Blotting of proteins 2.15 Evaluation of protein pherograms 2.16 Precast gels for protein electrophoresis. Rehydratable gels 3 Electrophoresis of nucleic acids 3.1 Agarose gel electrophoresis of nucleic acids 3.2 Pulsed-field gel electrophoresis of nucleic acids 3.3 Capillary electrophoresis of nucleic acids 3.4 Polyacrylamide gel electrophoresis of nucleic acids 3.5 Microchip electrophoresis of nucleic acids 3.6 Blotting of nucleic acids 3.7 Evaluation of nucleic acid pherograms 3.8 Precast gels for nucleic acid electrophoresis 4 Iontophoresis 5 History of electrophoresis and iontophoresis 6 Troubleshooting Problems Solution of problems Reagents for electrophoresis Recipes for electrophoresis solutions SI units and physical constants used in electrophoresis Electrophoresis terms Index