ENGLISH

The Mitoribosome: Methods and Protocols

By Antoni Barrientos; Flavia Fontanesi

Book information

Publisher
Springer Nature
Year
2023
ISBN
9781071631713, 9781071631706, 1071631713
Language
english
Format
EPUB
Filesize
32 MB (33746715 bytes)
Pages
346\0
Library
SoftArchive
Time added
2023-07-16 16:42:47

Description

This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to study mitochondrial mRNAs that are translated on mitoribosomes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Mitoribosome: Methods and Protocols, aims to be a comprehensive guide for researchers in the field.

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