Site-specific recombinases : methods and protocols

Description

This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field. Front Matter ....Pages i-xi Generating Genetically Modified Mice: A Decision Guide (Ivo J. Huijbers)....Pages 1-19 Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes (Colin E. J. Pritchard, Lona J. Kroese, Ivo J. Huijbers)....Pages 21-35 Building Cre Knockin Rat Lines Using CRISPR/Cas9 (Yuanwu Ma, Lianfeng Zhang, Xingxu Huang)....Pages 37-52 Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases (Eugenia Voziyanova, Rachelle P. Anderson, Yuri Voziyanov)....Pages 53-67 Use of the DICE (Dual Integrase Cassette Exchange) System (Alfonso P. Farruggio, Mital S. Bhakta, Michele P. Calos)....Pages 69-85 Ligand-Controlled Site-Specific Recombination in Zebrafish (Avinash Chekuru, Veronika Kuscha, Stefan Hans, Michael Brand)....Pages 87-97 Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre (Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, Michael T. Chin)....Pages 99-107 Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain (Jonathan C. Y. Tang, Stephanie Rudolph, Constance L. Cepko)....Pages 109-126 Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors (Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson et al.)....Pages 127-150 Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X (Lilya Kopertekh, Joachim Schiemann)....Pages 151-168 Nanoparticle-Mediated Recombinase Delivery into Maize (Susana Martin-Ortigosa, Brian G. Trewyn, Kan Wang)....Pages 169-180 Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines (Qi Lu, Zhuo-Hua Pan)....Pages 181-194 FLPing Genes On and Off in Drosophila (Bonnie M. Weasner, Jinjin Zhu, Justin P. Kumar)....Pages 195-209 Imaging Neural Architecture in Brainbow Samples (Douglas H. Roossien, Dawen Cai)....Pages 211-228 Purification and In Vitro Characterization of Zinc Finger Recombinases (Femi J. Olorunniji, Susan J. Rosser, W. Marshall Stark)....Pages 229-245 Preparing Mate-Paired Illumina Libraries Using Cre Recombinase (Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, Feng Chen)....Pages 247-261 Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination (Ze Peng, Jeff L. Froula, Jan-Fang Cheng)....Pages 263-284 Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways (Wei Liu, Laura R. Tuck, Jon Marles Wright, Yizhi Cai)....Pages 285-302 Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family (Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C. M. Smith, W. Marshall Stark, Sean D. Colloms)....Pages 303-323 Production of Minicircle DNA Vectors Using Site-Specific Recombinases (Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani, Mohammad Hossein Nasr-Esfahani)....Pages 325-339 Back Matter ....Pages 341-342