Transplantation of Chemically-Induced Pluripotent Stem Cell-Derived Islets under Abdominal Anterior Rectus Sheath in a Type 1 Diabetes Patient

Description

A paper written by Chinese scientists who claim to have reversed Type 1 diabetes in a patient. Transplantation of chemically induced pluripotent stem-cell-derived islets under abdominal anterior rectus sheath in a type ... Introduction Results Overview of study design Study participant Generation of patient CiPSC-islets Pre-transplant release of CiPSC-islets Transplantation of autologous CiPSC-islets Glycemic control Graft function Safety assessments Discussion Insulin independence and glycemic control Transplantation strategy Autologous chemically induced stem-cell-derived islets Limitations of the study Resource availability Lead contact Materials availability Data and code availability Acknowledgments Author contributions Declaration of interests Supplemental information References STAR★Methods Key resources table Experimental model and study participant details Method details Generation of CiPSCs from the patient's adipose-derived mesenchymal stromal cells Assessment of patient CiPSC clones Preparation of CiPSC-islets and safety assessment Whole genome/exome sequencing (WGS/WES) Single cell RNA-sequencing analysis Single-cell RNA pre-processing Integration with human pancreatic data Short-tandem repeat (STR) analysis Immunofluorescence Teratoma formation RT–qPCR analysis Transplantation in mouse model Transplantation into STZ-treated diabetic mice Transplantation into non-STZ-treated healthy mice Flow cytometry Glucose stimulated insulin secretion (GSIS) assay Cell preparation and subanterior rectus sheath transplantation Immunosuppression and other medications Laboratory methods Quantification and statistical analysis BETA-2 score calculation Additional resources