ENGLISH

Foodborne Bacterial Pathogens: Methods and Protocols

Book information

Publisher
Springer New York,Humana Press
Year
2019
ISBN
978-1-4939-8999-7, 978-1-4939-9000-9
Language
english
Format
PDF
Filesize
7 MB (7718938 bytes)
Series
Methods in Molecular Biology 1918
Edition
1st ed.
Pages
XI, 278\276
Time added
2019-01-12 07:29:58

Description

This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products (Eduardo Ximenes, Seockmo Ku, Lori Hoagland, Michael R. Ladisch)....Pages 3-20 Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens (Maggie R. Williams, Syed A. Hashsham)....Pages 21-33 The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens (Alejandro Garrido-Maestu, David Tomás Fornés, Marta Prado Rodríguez)....Pages 35-45 Sequence-Specific End Labeling of Oligonucleotides (SSELO)-Based Microbial Detection (Tanja Kostic, Levente Bodrossy)....Pages 47-56 Rapid On-Site Detection and Quantification of Foodborne Pathogens Using Microfluidic Devices (Nobuyasu Yamaguchi)....Pages 57-66 Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria (Matthew Dunne, Martin J. Loessner)....Pages 67-86 EIS-Based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes (Palmiro Poltronieri, Elisabetta Primiceri, Rajeswaran Radhakrishnan)....Pages 87-101 Front Matter ....Pages 103-103 Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions (Olivier Habimana)....Pages 105-116 Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopic Observation (Thomas Brauge, Graziella Midelet-Bourdin, Christophe Soumet)....Pages 117-128 Isolation of Bacterial RNA from Foods Inoculated with Pathogens (Deepti Tyagi, Autumn L. Kraft, Teresa M. Bergholz)....Pages 129-137 Use of Two-Dimensional Electrophoresis to Explore Foodborne Bacterial Pathogen Responses to Gastrointestinal Stress (Denise Schrama, Maria Leonor Faleiro)....Pages 139-147 Identification of Putative Biomarkers Specific to Foodborne Pathogens Using Metabolomics (Snehal R. Jadhav, Rohan M. Shah, Avinash V. Karpe, David J. Beale, Konstantinos A. Kouremenos, Enzo A. Palombo)....Pages 149-164 Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells (Florence Dubois-Brissonnet)....Pages 165-170 High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Structural Dynamic of Foodborne Pathogens (Alexis Canette, Julien Deschamps, Romain Briandet)....Pages 171-182 Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae (Macarena A. Varas, Javiera Ortíz-Severín, Andrés E. Marcoleta, Carlos A. Santiviago, Miguel L. Allende, Francisco P. Chávez)....Pages 183-190 Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection (Francisco P. Chávez, Andrea Calixto)....Pages 191-197 Front Matter ....Pages 199-199 Utilizing the Public GenomeTrakr Database for Foodborne Pathogen Traceback (Ruth E. Timme, Maria Sanchez Leon, Marc W. Allard)....Pages 201-212 Establishment of a Standardized 16S rDNA Library Preparation to Enable Analysis of Microbiome in Poultry Processing Using Illumina MiSeq Platform (Kristina M. Feye, Steven C. Ricke)....Pages 213-227 Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics (Arnaud Bridier)....Pages 229-245 Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments (Paw Dalgaard, Ole Mejlholm)....Pages 247-264 Network Biology Approaches to Identify Molecular and Systems-Level Differences Between Salmonella Pathovars (Marton Olbei, Robert A. Kingsley, Tamas Korcsmaros, Padhmanand Sudhakar)....Pages 265-273 Back Matter ....Pages 275-278

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