ENGLISH

Optical Fluorescence Microscopy: From the Spectral to the Nano Dimension

By Franco Quercioli (auth.) , Alberto Diaspro (eds.)

Book information

Publisher
Springer-Verlag Berlin Heidelberg
Year
2011
ISBN
978-3-642-15174-3, 978-3-642-15175-0
DOI
10.1007/978-3-642-15175-0
Language
english
Format
PDF
Filesize
5 MB (5487145 bytes)
Edition
1
Pages
244\251
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00
Biological Microscopy;Cell Biology;Biophysics andgeneral

Description

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

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