ENGLISH

Protein-Protein Interactions. Methods and Applications

By Haian Fu

Book information

Publisher
Humana Press
Year
2004
ISBN
9781588291202, 1588291200
Open Library ID
OL15559900M
Language
english
Format
RAR
Filesize
6 MB (6567965 bytes)
Series
Methods in Molecular Biology
Edition
1
Pages
544\0
Library
mexmat
Time added
2009-07-20 03:45:11

Description

A collection of highly successful biochemical, biophysical, genetic, and computational techniques for studying protein-protein interactions. These readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions in vitro or in vivo, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics and bioinformatics-based approaches, such as the Scansite program for computational analysis. These powerful tools are essential for deciphering how proteins interact with each other to form biological networks, as well as for unraveling protein-protein interactions in disease to find novel therapeutic targets.

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