ENGLISH

Mass Cytometry: Methods and Protocols

By Helen M. McGuire , Thomas M. Ashhurst

Book information

Publisher
Springer New York; Humana
Year
2019
ISBN
978-1-4939-9453-3;978-1-4939-9454-0
Language
english
Format
PDF
Filesize
14 MB (15165078 bytes)
Series
Methods in Molecular Biology 1989
Edition
1st ed.
Pages
XII, 338\333
Time added
2019-09-18 12:17:00
Life Sciences; Cell Biology; Mass Spectrometry; An

Description

This book details up-to-date and cutting edge compilation of protocols in mass cytometry. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Cytometry: Methods and Protocols aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xii Front Matter ....Pages 1-1 Setting Up Mass Cytometry in a Shared Resource Lab Environment (Sarah Warth, Désirée Kunkel)....Pages 3-11 Acquisition, Processing, and Quality Control of Mass Cytometry Data (Brian H. Lee, Adeeb H. Rahman)....Pages 13-31 Front Matter ....Pages 33-33 Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating (Amelia Au-Yeung, Chikara Takahashi, W. Rodney Mathews, William E. O’Gorman)....Pages 35-45 Method for Tagging Antibodies with Metals for Mass Cytometry Experiments (Stephen Gregory Chang, Cynthia J. Guidos)....Pages 47-54 Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis (Felix J. Hartmann, Erin F. Simonds, Nora Vivanco, Trevor Bruce, Luciene Borges, Garry P. Nolan et al.)....Pages 55-81 Titration of Mass Cytometry Reagents (Caryn van Vreden, Paula Niewold, Helen M. McGuire, Barbara Fazekas de St. Groth)....Pages 83-92 Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry (Axel Ronald Schulz, Henrik E. Mei)....Pages 93-108 Front Matter ....Pages 109-109 Automated Cell Processing for Mass Cytometry Experiments (Jaromir Mikes, Axel Olin, Tadepally Lakshmikanth, Yang Chen, Petter Brodin)....Pages 111-123 Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry (Lisa E. Wagar)....Pages 125-135 Front Matter ....Pages 137-137 Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry (Diana Shinko, Thomas M. Ashhurst, Helen M. McGuire, Kellie A. Charles)....Pages 139-146 Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry (Yannick Simoni, Michael Fehlings, Evan W. Newell)....Pages 147-158 Analysis of the Murine Bone Marrow Hematopoietic System Using Mass and Flow Cytometry (Thomas M. Ashhurst, Darren A. Cox, Adrian L. Smith, Nicholas J. C. King)....Pages 159-192 Mass Cytometric Cell Cycle Analysis (Gregory K. Behbehani)....Pages 193-215 Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry (Mikael Roussel, Todd Bartkowiak, Jonathan M. Irish)....Pages 217-226 Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry (Andrew J. Mitchell, Angela Ivask, Yi Ju)....Pages 227-241 Front Matter ....Pages 243-243 Data-Driven Flow Cytometry Analysis (Sherrie Wang, Ryan R. Brinkman)....Pages 245-265 Analysis of Mass Cytometry Data (Christina B. Pedersen, Lars R. Olsen)....Pages 267-279 Analysis of High-Dimensional Phenotype Data Generated by Mass Cytometry or High-Dimensional Flow Cytometry (Branko Cirovic, Natalie Katzmarski, Andreas Schlitzer)....Pages 281-294 Computational Analysis of High-Dimensional Mass Cytometry Data from Clinical Tissue Samples (Sam Norton, Roslyn Kemp)....Pages 295-307 Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery (Hannah G. Polikowsky, Katherine A. Drake)....Pages 309-332 Back Matter ....Pages 333-338

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