NADPH Oxidases: Methods and Protocols

Description

This detailed volume explores the NADPH oxidase family of enzymes in human physiology and genetic disease, in which early discoveries represent prime examples of the finest translational “from bed to bench and back” studies. Methods are included for testing assembly and function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS) generation in different systems by various means, while addressing pitfalls of ROS probes currently being used, as well as protocols on NADPH oxidase regulation and their function in cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, NADPH Oxidases: Methods and Protocols will aid researchers working with the NOX/DUOX family in continuing and expanding upon their vital research. Front Matter ....Pages i-xv Front Matter ....Pages 1-1 Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease (William M. Nauseef, Robert A. Clark)....Pages 3-16 Mammalian NADPH Oxidases (Hélène Buvelot, Vincent Jaquet, Karl-Heinz Krause)....Pages 17-36 Front Matter ....Pages 37-37 Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy (Algirdas J. Jesaitis, Marcia Riesselman, Ross M. Taylor, Susan Brumfield)....Pages 39-59 Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs) (Jennifer L. Meitzler)....Pages 61-74 A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase (Tania Bizouarn, Hager Souabni, Xavier Serfaty, Aicha Bouraoui, Rawand Masoud, Gilda Karimi et al.)....Pages 75-101 NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules (Fiona Augsburger, Delphine Rasti, Yves Cambet, Vincent Jaquet)....Pages 103-111 Spectroscopy of NOX Protein Family Members (Yoko Nakano, William M. Nauseef)....Pages 113-120 Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases (Hideki Sumimoto, Reiko Minakami, Kei Miyano)....Pages 121-137 Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays (Mary C. Dinauer)....Pages 139-151 The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis (Sylvain Beaumel, Marie José Stasia)....Pages 153-171 Functional Characterization of DUOX Enzymes in Reconstituted Cell Models (Agnieszka Korzeniowska, Ágnes P. Donkó, Stanislas Morand, Thomas L. Leto)....Pages 173-190 Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR (Becky A. Diebold, S. Garrett Wilder, Xavier De Deken, Jennifer L. Meitzler, James H. Doroshow, James W. McCoy et al.)....Pages 191-229 Front Matter ....Pages 231-231 Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells (Fiona Augsburger, Aleksandra Filippova, Vincent Jaquet)....Pages 233-241 HPLC-Based Monitoring of Oxidation of Hydroethidine for the Detection of NADPH Oxidase-Derived Superoxide Radical Anion (Jacek Zielonka, Monika Zielonka, Balaraman Kalyanaraman)....Pages 243-258 Visualization of Intracellular Hydrogen Peroxide with the Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells (Yulia G. Ermakova, Nataliya M. Mishina, Carsten Schultz, Vsevolod V. Belousov)....Pages 259-274 Imaging Intracellular H2O2 with the Genetically Encoded PerFRET and OxyFRET Probes (Balázs Enyedi, Miklós Geiszt)....Pages 275-282 Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae (Mark Jelcic, Balázs Enyedi, Philipp Niethammer)....Pages 283-299 Kinetic Analysis of Phagosomal ROS Generation (Sophie Dupré-Crochet, Marie Erard, Oliver Nüβe)....Pages 301-312 Imaging Intestinal ROS in Homeostatic Conditions Using L-012 (Emer Conroy, Gabriella Aviello)....Pages 313-327 Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo (Bejan J. Saeedi, Bindu Chandrasekharan, Andrew S. Neish)....Pages 329-337 Front Matter ....Pages 339-339 Phosphorylation of gp91phox/NOX2 in Human Neutrophils (Houssam Raad, Riad Arabi Derkawi, Asma Tlili, Sahra A. Belambri, Pham My-Chan Dang, Jamel El-Benna)....Pages 341-352 The Molecular Regulation and Functional Roles of NOX5 (David J. R. Fulton)....Pages 353-375 Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex (Edgar Pick)....Pages 377-415 Rational Design and Delivery of NOX-Inhibitory Peptides (Eugenia Cifuentes-Pagano, Patrick J. Pagano)....Pages 417-428 High-Throughput Screening of NOX Inhibitors (Jacek Zielonka, Monika Zielonka, Gang Cheng, Micael Hardy, Balaraman Kalyanaraman)....Pages 429-446 Protein–Protein Interaction Assay to Analyze NOX4/p22phox Heterodimerization (Sharon O’Neill, Ulla G. Knaus)....Pages 447-458 Front Matter ....Pages 459-459 Isolation of Redox-Active Endosomes (Redoxosomes) and Assessment of NOX Activity (Weam S. Shahin, John F. Engelhardt)....Pages 461-472 Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness (Howard E. Boudreau, Thomas L. Leto)....Pages 473-485 NADPH Oxidases and Aging Models of Lung Fibrosis (Karen Bernard, Victor J. Thannickal)....Pages 487-496 Proteomic Methods to Evaluate NOX-Mediated Redox Signaling (Christopher M. Dustin, Milena Hristova, Caspar Schiffers, Albert van der Vliet)....Pages 497-515 Neutrophil Extracellular Traps (Balázs Rada)....Pages 517-528 Front Matter ....Pages 529-529 Chronic Granulomatous Disease (Dirk Roos)....Pages 531-542 Diagnostic Testing for Chronic Granulomatous Disease (Douglas B. Kuhns)....Pages 543-571 Gastrointestinal Complications in Chronic Granulomatous Disease (E. Liana Falcone, Steven M. Holland)....Pages 573-586 Ex Vivo Models of Chronic Granulomatous Disease (Julie Brault, Bénédicte Vigne, Marie José Stasia)....Pages 587-622 Gene Editing in Chronic Granulomatous Disease (Colin L. Sweeney, Randall K. Merling, Suk See De Ravin, Uimook Choi, Harry L. Malech)....Pages 623-665 DUOX Defects and Their Roles in Congenital Hypothyroidism (Xavier De Deken, Françoise Miot)....Pages 667-693 NADPH Oxidases in Inflammatory Bowel Disease (Emily Stenke, Billy Bourke, Ulla G. Knaus)....Pages 695-713 Correction to: NADPH Oxidases in Inflammatory Bowel Disease (Emily Stenke, Billy Bourke, Ulla G. Knaus)....Pages C1-C1 Back Matter ....Pages 715-718