ENGLISH

Brain Development: Methods and Protocols

By Simon G. Sprecher

Book information

Publisher
Springer New York;Humana
Year
2020
ISBN
978-1-4939-9731-2, 978-1-4939-9732-9
Language
english
Format
PDF
Filesize
20 MB (21398716 bytes)
Series
Methods in Molecular Biology 2047
Edition
2nd ed. 2020
Pages
XIV, 527\520
Time added
2020-02-08 04:40:58
Life Sciences; Neurobiology; Developmental Biology

Description

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain. Front Matter ....Pages i-xiv Front Matter ....Pages 1-1 Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra (Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, Brigitte Galliot)....Pages 3-24 Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis (Jamie A. Havrilak, Michael J. Layden)....Pages 25-43 Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis (Fabian Rentzsch, Eduard Renfer, Ulrich Technau)....Pages 45-57 Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System (Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez)....Pages 59-80 Immunostaining of the Embryonic and Larval Drosophila Brain (Frank Hirth, Danielle C. Diaper)....Pages 81-96 Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila (David Jussen, Rolf Urbach)....Pages 97-113 Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling (Karoline F. Kraft, Rolf Urbach)....Pages 115-135 Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update (Emma L. Powell, Iris Salecker)....Pages 137-152 Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain (Karolina Miszczak, Boris Egger)....Pages 153-160 CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila (Cornelia Fritsch, Simon G. Sprecher)....Pages 161-189 The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development (Max S. Farnworth, Kolja N. Eckermann, Hassan M. M. Ahmed, Dominik S. Mühlen, Bicheng He, Gregor Bucher)....Pages 191-217 A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum (Marita Buescher, Georg Oberhofer, Natalia Carolina Garcia-Perez, Gregor Bucher)....Pages 219-232 Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium (Vera S. Hunnekuhl, Janna Siemanowski, Max S. Farnworth, Bicheng He, Gregor Bucher)....Pages 233-251 X-Ray Microscopy of the Larval Crustacean Brain (Jakob Krieger, Franziska Spitzner)....Pages 253-270 Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain (Steffen Harzsch, Caroline Viertel)....Pages 271-291 Immunostainings in Nervous System Development of the Nematode C. elegans (Janet S. Duerr)....Pages 293-310 Methods in Brain Development of Molluscs (Andreas Wanninger, Tim Wollesen)....Pages 311-324 A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization (Clare Hudson)....Pages 325-345 Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum (Yann Le Petillon, Stéphanie Bertrand, Héctor Escrivà)....Pages 347-359 Front Matter ....Pages 361-361 In Situ Hybridization and Immunostaining of Xenopus Brain (Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, Rong-qiao He)....Pages 363-375 Morpholino Studies in Xenopus Brain Development (Jennifer E. Bestman, Hollis T. Cline)....Pages 377-395 Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish (Gilbert Lauter, Iris Söll, Giselbert Hauptmann)....Pages 397-409 Live Morphometric Classification of Sensory Neurons in Larval Zebrafish (Gema Valera, Hernán López-Schier)....Pages 411-419 Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain (Richard P. Tucker, Tatsuto Ishimaru, Qizhi Gong)....Pages 421-437 Gene Silencing in Chicken Brain Development (Georgia Tsapara, Irwin Andermatt, Esther T. Stoeckli)....Pages 439-456 Transplantation of Neural Tissue: Quail–Chick Chimeras (Andrea Streit, Claudio D. Stern)....Pages 457-473 Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development (Jinling Liu, Aimin Liu)....Pages 475-489 The Cre/Lox System to Assess the Development of the Mouse Brain (Claudius F. Kratochwil, Filippo M. Rijli)....Pages 491-512 In Utero Electroporation to Study Mouse Brain Development (Emilie Pacary, François Guillemot)....Pages 513-523 Back Matter ....Pages 525-527

Similar books

Session C11: Ancient Cultural Landscapes in South Europe – their Ecological Setting and Evolution, Session C22: Gardeners from South America, Session S04: Agro-Pastoralism and Early Metallurgy Sessions, Session WS29: The Idea of Enclosure in Recent Iberian Prehistory, Session C88: Rhytmes et causalites des dynamiques de l'anthropisation en Europe entre 6500 ET 500 BC: Hypotheses socio-culturelles et/ou climatiques: Proceedings of the XV UISPP World Congress (Lisbon 4-9 September 2006) / Actes du XV Congrès Mondial (Lisbonne 4-9 Septembre 2006) Vol.36

Session C11: Ancient Cultural Landscapes in South Europe – their Ecological Setting and Evolution, Session C22: Gardeners from South America, Session S04: Agro-Pastoralism and Early Metallurgy Sessions, Session WS29: The Idea of Enclosure in Recent Iberian Prehistory, Session C88: Rhytmes et causalites des dynamiques de l'anthropisation en Europe entre 6500 ET 500 BC: Hypotheses socio-culturelles et/ou climatiques: Proceedings of the XV UISPP World Congress (Lisbon 4-9 September 2006) / Actes du XV Congrès Mondial (Lisbonne 4-9 Septembre 2006) Vol.36

2010 · PDF

THE BRITISH ARMY IN INDIA: ITS PRESERVATION BY AN APPROPRIATE CLOTHING, HOUSING, LOCATING, RECREATIVE EMPLOYMENT, AND HOPEFUL ENCOURAGEMENT OF THE TROOPS. with AN APPENDIX ON INDIA : THE CLIMATE OP ITS HILLS ; THE DEVELOPMENT OF ITS RESODRCBS, INDUSTRY, AND ARTS ; THE ADMINISTRATION OF JUSTICE ; THE BLACK ACT ; THE PROGRESS OF CHRISTIANITY ; THE TRAFFIC IN OPIUM ; THE VALUE OF INDIA ; PERMANENT CAUSES OF DISAFFECTION, AND OF THE RECENT REBELLION ; THE TRADITIONARY POLICY; MISGOVERNMENT BY NATIVE RULERS ; ANNEXATIONS OF THEIR TERRITORY, ETC.

THE BRITISH ARMY IN INDIA: ITS PRESERVATION BY AN APPROPRIATE CLOTHING, HOUSING, LOCATING, RECREATIVE EMPLOYMENT, AND HOPEFUL ENCOURAGEMENT OF THE TROOPS. with AN APPENDIX ON INDIA : THE CLIMATE OP ITS HILLS ; THE DEVELOPMENT OF ITS RESODRCBS, INDUSTRY, AND ARTS ; THE ADMINISTRATION OF JUSTICE ; THE BLACK ACT ; THE PROGRESS OF CHRISTIANITY ; THE TRAFFIC IN OPIUM ; THE VALUE OF INDIA ; PERMANENT CAUSES OF DISAFFECTION, AND OF THE RECENT REBELLION ; THE TRADITIONARY POLICY; MISGOVERNMENT BY NATIVE RULERS ; ANNEXATIONS OF THEIR TERRITORY, ETC.

1858 · PDF

Idries Shah 27 Books Collection : A Perfumed Scorpion, A Veiled Gazelle, Caravan of Dreams, Darkest England, Destination Mecca, Evenings with Idries Shah, Knowing How to Know, Learning How to Learn, Letters and Lectures of Idries Shah, Neglected aspects of Sufi study, Observations, Oriental Magic, Reflections, Seeker after Truth, Special Illumination, Special Problems in the study of Sufi ideas, Sufi thought and action, Tales of the Dervishes, The Dermis Probe, The Elephant in the Dark, The Englishman Handbook, Idries Shah Antology, The Magic Monastery, The natives are restless, wisdom of the Idiots PDF.

Idries Shah 27 Books Collection : A Perfumed Scorpion, A Veiled Gazelle, Caravan of Dreams, Darkest England, Destination Mecca, Evenings with Idries Shah, Knowing How to Know, Learning How to Learn, Letters and Lectures of Idries Shah, Neglected aspects of Sufi study, Observations, Oriental Magic, Reflections, Seeker after Truth, Special Illumination, Special Problems in the study of Sufi ideas, Sufi thought and action, Tales of the Dervishes, The Dermis Probe, The Elephant in the Dark, The Englishman Handbook, Idries Shah Antology, The Magic Monastery, The natives are restless, wisdom of the Idiots PDF.

2022 · PDF

The travels of Capts. Lewis and Clarke from St. Louis, by way of the Missouri and Columbia rivers, to the Pacific ocean; performed in the years 1804, 1805 & 1806, by order of the government of the United States. Containing delineations of the manners, customs, religion, &c. of the Indians, comp. from various authentic sources, and original documents, and a summary of the Statistical view of the Indian nations, from the official communication of Meriwether Lewis. Illustrated with a map of the country, inhabited by the western tribes of Indians

The travels of Capts. Lewis and Clarke from St. Louis, by way of the Missouri and Columbia rivers, to the Pacific ocean; performed in the years 1804, 1805 & 1806, by order of the government of the United States. Containing delineations of the manners, customs, religion, &c. of the Indians, comp. from various authentic sources, and original documents, and a summary of the Statistical view of the Indian nations, from the official communication of Meriwether Lewis. Illustrated with a map of the country, inhabited by the western tribes of Indians

1809 · PDF

Professional Linux kernel architecture ''Wrox programmer to programmer''--Cover. - ''What you are reading right now is the result of an evolution over more than seven years: After two years of writing, the first edition was published in German by Carl Hanser Verlag in 2003. It then described kernel 2.6.0. The test was used as a basis for the low-level design documentation for the EAL4+ security evaluation of Red Hat Enterprise Linux 5, requiring to update it to kernel 2.6.18 (if the EAL acronym does not mean anything to you, then Wikipedia is once more your friend). Hewlett-Packard sponsored the translation into English and has, thankfully, granted the rights to publish the result. Updates to kernel 2.6.24 were then performed specifically for this book''--P. ix

Professional Linux kernel architecture ''Wrox programmer to programmer''--Cover. - ''What you are reading right now is the result of an evolution over more than seven years: After two years of writing, the first edition was published in German by Carl Hanser Verlag in 2003. It then described kernel 2.6.0. The test was used as a basis for the low-level design documentation for the EAL4+ security evaluation of Red Hat Enterprise Linux 5, requiring to update it to kernel 2.6.18 (if the EAL acronym does not mean anything to you, then Wikipedia is once more your friend). Hewlett-Packard sponsored the translation into English and has, thankfully, granted the rights to publish the result. Updates to kernel 2.6.24 were then performed specifically for this book''--P. ix

2008 · PDF