Animal Cell Biotechnology: Methods and Protocols

Description

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. Front Matter ....Pages i-xiv Front Matter ....Pages 1-1 Near-Physiological Cell Cycle Synchronization with Countercurrent Centrifugal Elutriation (Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, Uwe Jandt)....Pages 3-16 Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays (Oliver Wehmeier, Alexander Loa)....Pages 17-25 Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment (Patrick Mayrhofer, Renate Kunert)....Pages 27-39 Front Matter ....Pages 41-41 ambr® 15 Microbioreactors for CHO Cell Cultivation (Steve R. C. Warr)....Pages 43-67 Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications (Vincent Wiegmann, Cristina Bernal Martinez, Frank Baganz)....Pages 69-81 HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR) (Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina)....Pages 83-103 Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode (Tim Bürgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl et al.)....Pages 105-123 Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors (Moritz Wolf, Massimo Morbidelli)....Pages 125-140 Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production (Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, Udo Reichl)....Pages 141-168 How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale (Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, Dieter Eibl)....Pages 169-186 Front Matter ....Pages 187-187 Generic Workflow for the Setup of Mechanistic Process Models (Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, Christoph Herwig)....Pages 189-211 Estimation of Process Model Parameters (Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández Rodríguez, Kim B. Kuchemüller, Johannes Möller et al.)....Pages 213-234 Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments (Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller)....Pages 235-249 Design, Optimization, and Adaptive Control of Cell Culture Seed Trains (Tanja Hernández Rodríguez, Björn Frahm)....Pages 251-267 Front Matter ....Pages 269-269 High-Throughput Quantification and Glycosylation Analysis of Antibodies Using Bead-Based Assays (Sebastian Giehring)....Pages 271-284 Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture (Olivier Henry, Eric Karengera, Florian Cambay, Gregory De Crescenzo)....Pages 285-293 Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells (Julia Hennicke, Renate Kunert)....Pages 295-302 Raman Trapping Microscopy for Non-invasive Analysis of Biological Samples (Hesham K. Yosef, Karin Schütze)....Pages 303-317 An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures (Mario Lederle, Mircea Tric, Claudio Packi, Tobias Werner, Philipp Wiedemann)....Pages 319-333 Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures (Lukas Käßer, Jan Zitzmann, Tanja Grein, Tobias Weidner, Denise Salzig, Peter Czermak)....Pages 335-364 Front Matter ....Pages 365-365 Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method (Ricardo J. S. Silva, João P. Mendes, Manuel J. T. Carrondo, Paula M. Marques, Cristina Peixoto)....Pages 367-384 Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System (Gavin McGauran, Sara Linse, David J. O’Connell)....Pages 385-396 Back Matter ....Pages 397-399