Antibiotic Resistance Protocols

Description

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response. New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-x Methods for Measuring the Production of Quorum Sensing Signal Molecules (Manuel Alcalde-Rico, José Luis Martínez)....Pages 1-15 Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics (Gareth McVicker, Tomasz K. Prajsnar, Simon J. Foster)....Pages 17-27 Method for Detecting and Studying Genome-Wide Mutations in Single Living Cells in Real Time (Marina Elez, Lydia Robert, Ivan Matic)....Pages 29-39 Detecting Phenotypically Resistant Mycobacterium tuberculosis Using Wavelength Modulated Raman Spectroscopy (Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie)....Pages 41-50 A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics (Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, Joanna Bacon)....Pages 51-57 Application of Continuous Culture for Assessing Antibiotic Activity Against Mycobacterium tuberculosis (Charlotte L. Hendon-Dunn, Saba Anwar, Christopher Burton, Joanna Bacon)....Pages 59-73 Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing (Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, Tom Olesen)....Pages 75-84 Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria (Robert J. H. Hammond, Vincent O. Baron, Sam Lipworth, Stephen H. Gillespie)....Pages 85-94 Methods to Determine Mutational Trajectories After Experimental Evolution of Antibiotic Resistance (Douglas L. Huseby, Diarmaid Hughes)....Pages 95-103 Selection of ESBL-Producing E. coli in a Mouse Intestinal Colonization Model (Frederik Boëtius Hertz, Karen Leth Nielsen, Niels Frimodt-Møller)....Pages 105-115 Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa (Leticia Muraro Wildner, Katherine A. Gould, Simon J. Waddell)....Pages 117-128 Direct in Gel Genomic Detection of Antibiotic Resistance Genes in S1 Pulsed Field Electrophoresis Gels (Mark A. Toleman)....Pages 129-136 Using RT qPCR for Quantifying Mycobacteria marinum from In Vitro and In Vivo Samples (Han Xaio, Stephen H. Gillespie)....Pages 137-145 Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics (Tomasz K. Prajsnar, Gareth McVicker, Alexander Williams, Stephen A. Renshaw, Simon J. Foster)....Pages 147-156 A Method to Evaluate Persistent Mycobacterium tuberculosis In Vitro and in the Cornell Mouse Model of Tuberculosis (Yanmin Hu, Anthony Coates)....Pages 157-166 Back Matter ....Pages 167-168