Atomic Force Microscopy in Molecular and Cell Biology

Description

The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc. Front Matter ....Pages i-xiii Principles of Atomic Force Microscopy (Wanxin Sun)....Pages 1-28 Atomic Force Microscopy-Based Single Molecule Force Spectroscopy for Biological Application (Chao Tang, Youjie Fan, Junhong Lü)....Pages 29-40 In Situ Single Molecule Detection on Cell Membrane and Label Molecule Distributions Using AFM/NSOM (Jiang Pi, Hua Jin, Jiye Cai)....Pages 41-54 AFM Imaging-Force Spectroscopy Combination for Molecular Recognition at the Single-Cell Level (Filomena A. Carvalho, Nuno C. Santos)....Pages 55-76 Atomic Force Microscopy: A Nanoscopic Application in Molecular and Cell Biology (Huai-Hong Cai, Xueyi Zeng, Xiao Tang, Jiye Cai)....Pages 77-103 The Hyphenated Technique of High Speed Atomic Force Microscopy and Super Resolution Optical Detection System (Xiao Feng, Yunchang Guo, Hongjie An, Hongshun Yang)....Pages 105-130 AFM and NSOM/QD Based Direct Molecular Visualization at the Single-Cell Level (Liyun Zhong, Jiye Cai, Zhengwei Chen)....Pages 131-145 Assessment of Pathological or Drug-Dependent Changes in Cell Membrane Morphology and Cell Biomechanical Properties by Atomic Force Microscopy (Hua Jin, Yue Zhao, Wandang Wang, Jinhuan Jiang, Jiye Cai, Colin E. Evans)....Pages 147-160 In Situ Measuring Mechanical Properties of Normal and Disease Cells (Sui-Ping Deng, Yi-Li Yang, Xing-Xing Cheng, Wen-Rong Li, Jiye Cai)....Pages 161-178 High Resolution AFM and Its Applications (Hao Sun, Ming Ye, Wanxin Sun)....Pages 179-235