Spectroscopic membrane probes. Volume 2

Description

The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls. Read more... Abstract: The optical spectra of molecular membrane probes can be interpreted terms of the structure, dynamics, and the physiological state of the membrane. The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably, emerged directly from probe studies over the last 20 years. This work is designed to make these techniques accessible to a broad audience of cell biologist. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on required apparatus, experimental design, data analysis, and potential pitfalls Content: Cover Title Page Copyright Page DEDICATION ACKNOWLEDGMENTS PREFACE THE EDITOR CONTRIBUTORS Table of Contents Chapter 9: Fluorescence Correlation Spectroscopy and Photobleaching Recovery: Measurement of Transport and Chemical Kinetics Chapter 10: Cell Surface Clustering and Mobility of the Liganded LDL Receptor Measured by Digital Video Fluorescence Microscopy Chapter 11: Total Internal Reflection Fluorescence: Theory and Applications at Biosurfaces Chapter 12: Near-Field Imaging of Fluorescence Chapter 13: Fluorescent Probes of Anion Transport Inhibition in the Human Red Cell MembraneChapter 14: How to Choose a Potentiometric Membrane Probe Chapter 15: Potential-Sensitive Molecular Probes in Energy-Transducing Organelles Index