Biotechnology: A Laboratory Skills Course
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Chapter 1: The Biotechnology Industry What Is Biotechnology? Who Uses Biotechnology? Biotechnology Industry and Research Governmental Regulation of Biotechnology Industry Practices Careers in Biotechnology Chapter 1 Essay Questions Additional Resources Chapter 1 Vignettes Bioethics Biotechnology: Good or Bad? Biotech In The Real World: Bio-Rad: Then and Now How To... Write an SOP Careers In Biotech:Annabelle Tinoco, Quality Assurance Manager Chapter 2: Laboratory Skills Laboratory Safety Laboratory Notebooks Laboratory Equipment Numerical Data Significant Figures Scientific Notation Units of Measure Preparing Solutions Percent Solutions Using Stock Solutions Given in Terms of “x” Molar Solutions Chapter 2 Essay Questions Additional Resources Chapter 2 Laboratory Activities Activity 2.1 DNA Extraction and Precipitation Activity 2.2 Pipetting Activity 2.3 Kool-Aid Column Chromatography Activity 2.4 Making Solutions Activity 2.5 Titration Activity 2.6 Writing a Standard Operating Procedure Chapter 2 Extension Activities Chapter 2 Vignettes Bioethics: Waste Disposal Biotech In The Real World: Who Invented Claritin? How Laboratory Notebooks Played Their Part Careers In Biotech:Katie Dalpozzo, Laboratory Research Associate How To... Use an Adjustable-Volume Micropipet Chapter 3: Microbiology and Cell Culture Microbiology and Cell Biology Three Domains of Life Microorganisms and History Bacteria Uses of Bacteria in Biotechnology Culturing Bacteria in the Laboratory Microbiological Techniques Eukaryotic Cells Uses of Eukaryotic Cells in Biotechnology Eukaryotic Cell Culture Chapter 3 Essay Questions Additional Resources Chapter 3 Laboratory Activities Activity 3.1 Making Microbiology Media Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test） Activity 3.3 Microbes and Health:An Illustration of Koch's Postulates Activity 3.4 Gram Staining Activity 3.5 Quantifying Bacterial Numbers Activity 3.6 Staining Eukaryotic Cells Chapter 3 Extension Activities Chapter 3 Vignettes How To... Use Aseptic Technique to Transfer Bacteria Careers In Biotech:Mihaela Sabau, Clinical Laboratory Scientist Bioethics:Should Human Embryos Be Used for Research? Biotech In The Real World: Artificial Life! Chapter 4: DNA Structure and Analysis Molecular Biology DNA Structure Recombinant DNA Technology Restriction Enzymes Ligases Horizontal Agarose Gel Electrophoresis Restriction Enzymes as Forensic Tools Restriction Fragment Length Polymorphisms Chapter 4 Essay Questions Additional Resources Chapter 4 Laboratory Activities Activity 4.1 Restriction Site Prediction Using NEBcutter Activity 4.2 Casting Agarose Gels Activity 4.3 Dye Electrophoresis Activity 4.4 Restriction Digestion and Analysis of Lambda DNA Activity 4.5 Forensic DNA Fingerprinting Activity 4.6 Plasmid Mapping Chapter 4 Extension Activities Chapter 4 Vignettes Bioethics: Personal Genetic Information Careers In Biotech: Charles Lo, Commercial Rotation Development Program Associate How To... Set Up a Restriction Digest Biotech In The Real World: Did the Baker Do It? Chapter 5: Bacterial Transformation and Plasmid Purification History of Bacterial Transformation and Plasmids Plasmids: Structure and Function Using Plasmids in Biotechnology Transcriptional Regulation of Plasmids Transforming Cells Selection of Transformed Cells Plasmid Purification DNA Quantitation Chapter 5 Essay Questions Additional Resources Chapter 5 Laboratory Activities Activity 5.1 Bacterial Transformation with S3 Plasmid Activity 5.2 Bacterial Transformation withpGLO Plasmid Activity 5.3 Purification of S3 and pGLO Plasmids Activity 5.4 DNA Quantitation Chapter 5 Extension Activities Chapter 5 Vignettes Bioethics: A World without Antibiotics? How To... Transform E. coli Using Electroporation Biotech In The Real World:Plasmids and the Search for Energy How To... Use a Microcentrifuge Careers In Biotech: Denise Gangadharan,Acting Associate Director for Science Chapter 6: The Polymerase Chain Reaction Invention of PCR What Is PCR? PCR and DNA Replication Thermal Cyclers Types of PCR PCR Optimization Techniques Based on PCR Real-World Applications of PCR Chapter 6 Essay Questions Additional Resources Chapter 6 Laboratory Activities Activity 6.1 STR PCR Analysis Activity 6.2 GMO Detection by PCR Activity 6.3 Detection of the Human PV92 Alu Insertion Chapter 6 Extension Activities Chapter 6 Vignettes Careers In Biotech: Stan Hitomi,Math and Science Coordinator and Principal How To... Program a Thermal Cycler Biotech In The Real World: Biotech on the Pharm Bioethics: Forensic DNA Databases—Is Your Privacy Protected? Chapter 7: Protein Structure and Analysis Protein Synthesis Protein Structure Proteins in Biology Proteins in Biotechnology Methods of Protein Analysis Protein Quantitation Protein Electrophoresis Protein Chromatography Bioinformatics Chapter 7 Essay Questions Additional Resources Chapter 7 Laboratory Activities Activity 7.1 Protein Quantitation Using the Bradford Assay Activity 7.2 Size Exclusion Chromatography Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography Activity 7.4 SDS-PAGE of Fish Muscle Activity 7.5 Biofuel Enzyme Assay Activity 7.6 Exploring Bioinformatics with GFP Chapter 7 Extension Activities Chapter 7 Vignettes Bioethics: Food or Fuel? Careers In Biotech: Scott Chilton, Graduate Student How To... Set Up a Vertical Electrophoresis System Biotech In The Real World: Vaccines in Bananas Chapter 8: Immunological Applications The Immune System Antibodies Antibodies as Tools Immunoassays Enzyme-Linked Immunosorbent Assay （ELISA） Western Blotting Chapter 8 Essay Questions Additional Resources Chapter 8 Laboratory Activities Activity 8.1 Ouchterlony Double Immunodiffusion Assay Activity 8.2 Serum Antibody Detection by ELISA Activity 8.3 Quantitative ELISA Activity 8.4 Western Blotting Chapter 8 Extension Activities Chapter 8 Vignettes How To... Select an Antibody Bioethics: Treatment of HIV Positive People in the Developing World Biotech In The Real World:Using FACS to Diagnose and Treat Cancer Careers In Biotech:Amanda Williams, Clinical Lab Technician Chapter 9: Research Projects What Is Research? Student Research Projects Choosing a Research Project Planning a Project Experimental Design Data Analysis Communicating Research Project Ideas Research Tools and Techniques Agricultural and Animal Research Food Science Humans and Health Forensics Energy and the Environment Considerations and Tips for All Laboratory Projects Chapter 9 Essay Questions Additional Resources Chapter 9 Vignettes Careers In Biotech: Sophy Wong, HIV Specialist Bioethics: The Case of Woo Suk Hwang Biotech In The Real World: Abraham Lincoln High School Takes On the iGEM Challenge How To... Read a Journal Article Appendices Appendix A: Fast Gel Protocol Appendix B: Alternative Staining Methods for Agarose Gels Appendix C:Using the SmartSpec Plus Spectrophotometer Appendix D: Glossary Appendix E: Laboratory Skills Assessment Rubric Appendix F: Laboratory Notebook and Project Rubrics Laboratory Notebook Rubric Project Progress Rubric Project Proposal Rubric Presentation Content Rubric Presentation Style Rubric Appendix G: Trademarks and Legal Notices
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