ENGLISH

The Genetic Manipulation of Staphylococci: Methods and Protocols

By Jeffrey L. Bose

Book information

Publisher
Humana Press
Year
2015
ISBN
1493931571, 9781493931576
DOI
10.1007/978-1-4939-3158-3
ISSN
1064-3745
LCC
2015951972
Language
english
Format
PDF
Filesize
3 MB (3338634 bytes)
Series
Methods in Molecular Biology 1373
Pages
x+186\197
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-10-27 17:09:10
Microbiology; Gene Expression

Description

This detailed book contains chapters using unbiased approaches such as chemical and transposon mutagenesis, as well as a protocol for allelic exchange, to make targeted mutants in Staphylococcus, as generating mutants of this bacteria can be accomplished in many ways, depending on the intended changes. Using these methods, the authors have been successful at making mutants that span individual single-nucleotide changes in the chromosome to whole genome mutant libraries. The latter strategy provides a useful tool for high-throughput screening, while single nucleotide changes are an elegant way of teasing apart the importance of single nucleotides in gene expression or to create specific amino acid substitutions to examine protein function. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.

Similar books