Mass spectrometry in biophysics: conformation and dynamics of biomolecules

Description

"Recent advances in mass spectrometry (MS) have pushed the frontiers of analytical chemistry into the biophysical laboratory. As a result, the biophysical community's acceptance of MS-based methods, used to study protein higher-order structure and dynamics, has accelerated the expansion of biophysical MS." "Despite this growing trend, until now no single text has presented the full array of MS-based experimental techniques and strategies for biophysics. Mass Spectrometry in Biophysics expertly closes this gap in the literature."."Covering the theoretical background and technical aspects of each method, this much-needed reference offers an unparalleled overview of the current state of biophysical MS. Mass Spectrometry in Biophysics begins with a helpful discussion of general biophysical concepts and MS-related techniques." "Fully referenced and containing a helpful appendix on the physics of electrospray mass spectrometry, Mass Spectrometry in Biophysics also offers a compelling look at the current challenges facing biomolecular MS and the potential applications that will likely shape its future."--BOOK JACKET. Read more... Content: General overview of basic concepts in molecular biophysics. -- Overview of "traditional" experimental arsenal to study biomolecular structure and dynamics. -- Overview of biological mass spectrometry. -- Mass spectrometry-based approaches to study biomolecular higher-order structure. -- Mass spectrometry-based approaches to study biomolecular dynamics equilibrium intermediates. -- Kinetic studies by mass spectrometry. -- Protein interaction: a closer look at "structure-dynamics-function" triad. -- Synergism between biophysical techniques. -- Other biopolymers and synthetic polymers of biological interest. -- Biomolecular ions in a solvent-free environment. -- Mass spectrometry on the march: where next? From molecular biophysics to structural biology, perspectives and challenges. -- Appendix: Physics of electrospray. Abstract: "Recent advances in mass spectrometry (MS) have pushed the frontiers of analytical chemistry into the biophysical laboratory. As a result, the biophysical community's acceptance of MS-based methods, used to study protein higher-order structure and dynamics, has accelerated the expansion of biophysical MS." "Despite this growing trend, until now no single text has presented the full array of MS-based experimental techniques and strategies for biophysics. Mass Spectrometry in Biophysics expertly closes this gap in the literature."."Covering the theoretical background and technical aspects of each method, this much-needed reference offers an unparalleled overview of the current state of biophysical MS. Mass Spectrometry in Biophysics begins with a helpful discussion of general biophysical concepts and MS-related techniques." "Fully referenced and containing a helpful appendix on the physics of electrospray mass spectrometry, Mass Spectrometry in Biophysics also offers a compelling look at the current challenges facing biomolecular MS and the potential applications that will likely shape its future."--BOOK JACKET