ENGLISH

Protein Methods

By Daniel M Bollag , Michael D Rozycki , Stuart J Edelstein

Book information

Publisher
Wiley-Liss
Year
1996
ISBN
0471118370, 9780471118374
Language
english
Format
PDF
Filesize
111 MB (116628458 bytes)
Edition
2
Pages
432\431
Topic
Biology Molecular
Orientation
yes
Paginated
no
Scanned
yes
Time added
2025-01-24 22:03:16

Description

Protein Methods by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein Reviews from the first edition ...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time. --Biotechnology and Applied Biochemistry The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book... --Theoretical & Applied Genetics ...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price. --FEBS Letters This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization. Chapters include * Preparation for Protein Isolation * Protein Extraction and Solubilization * Protein Concentration Determination * Concentrating Protein Solutions * Gel Electrophoresis Under Denaturing Conditions * Gel Electrophoresis Under ...and feature * reagent items and equipment required for each method * chapter view-at-a-glance tabs * illustrations for all gel applications and other methods * complete supplier list * appendices of basic chemical parameters and molecular data * key references * descriptive tables * Safety notes.

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