ENGLISH

Expression, Purification, and Structural Biology of Membrane Proteins (Methods in Molecular Biology, 2127)

By Camilo Perez (editor) , Timm Maier (editor)

Book information

Publisher
Springer
Year
2020
ISBN
1071603728, 9781071603727
Language
english
Format
PDF
Filesize
12 MB (12574110 bytes)
Pages
437\424
Time added
2024-04-07 19:53:10

Description

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.

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