High Content Screening: A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

Description

This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field. Front Matter ....Pages i-xiii Front Matter ....Pages 1-1 Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays (Roy Edward)....Pages 3-19 General Staining and Segmentation Procedures for High Content Imaging and Analysis (Kevin M. Chambers, Bhaskar S. Mandavilli, Nick J. Dolman, Michael S. Janes)....Pages 21-31 Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis (Bhaskar S. Mandavilli, Robert J. Aggeler, Kevin M. Chambers)....Pages 33-46 Cell-Based High Content Analysis of Cell Proliferation and Apoptosis (Bhaskar S. Mandavilli, Michelle Yan, Scott Clarke)....Pages 47-57 Tools to Measure Autophagy Using High Content Imaging and Analysis (Nick J. Dolman, Brent A. Samson, Kevin M. Chambers, Michael S. Janes, Bhaskar S. Mandavilli)....Pages 59-71 Front Matter ....Pages 73-73 Guidelines for Microplate Selection in High Content Imaging (Oscar J. Trask)....Pages 75-88 Quality Control for High-Throughput Imaging Experiments Using Machine Learning in Cellprofiler (Mark-Anthony Bray, Anne E. Carpenter)....Pages 89-112 High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening (Steven A. Haney)....Pages 113-130 Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays (K. Kozak, B. Rinn, O. Leven, M. Emmenlauer)....Pages 131-148 Live-Cell High Content Screening in Drug Development (Milan Esner, Felix Meyenhofer, Marc Bickle)....Pages 149-164 Challenges and Opportunities in Enabling High-Throughput, Miniaturized High Content Screening (Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O’Connell, Martyn Banks, Andrea Weston)....Pages 165-191 Front Matter ....Pages 193-193 Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells (Verena Fetz, Roland H. Stauber, Shirley K. Knauer)....Pages 195-210 High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions (Yun Hua, Daniel P. Camarco, Christopher J. Strock, Paul A. Johnston)....Pages 211-227 High Content Imaging Assays for IL-6-Induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines (Paul A. Johnston, Malabika Sen, Yun Hua, Daniel P. Camarco, Tong Ying Shun, John S. Lazo et al.)....Pages 229-244 Single Cell and Population Level Analysis of HCA Data (David Novo, Kaya Ghosh, Sean Burke)....Pages 245-266 Utilization of Multidimensional Data in the Analysis of Ultra-High-Throughput High Content Phenotypic Screens (Judith Wardwell-Swanson, Yanhua Hu)....Pages 267-290 Front Matter ....Pages 291-291 High Content Screening of Mammalian Primary Cortical Neurons (Dario Motti, Murray Blackmore, John L. Bixby, Vance P. Lemmon)....Pages 293-304 Human-Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications (Joshua A. Harrill)....Pages 305-338 Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays (Oksana Sirenko, Evan F. Cromwell)....Pages 339-354 The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-Well Ultra-Low Attachment Microplates (David A. Close, Daniel P. Camarco, Feng Shan, Stanton J. Kochanek, Paul A. Johnston)....Pages 355-369 An Endothelial Cell/Mesenchymal Stem Cell Coculture Cord Formation Assay to Model Vascular Biology In Vitro (Michelle Swearingen, Beverly Falcon, Sudhakar Chintharlapalli, Mark Uhlik)....Pages 371-382 High-Throughput Automated Chemical Screens in Zebrafish (Manush Saydmohammed, Michael Tsang)....Pages 383-393 Back Matter ....Pages 395-397