ENGLISH

Genetic modification of hematopoietic stem cells: methods and protocols

By Guillermo Guenechea , Jose C. Segovia (auth.) , Christopher Baum (eds.)

Book information

Publisher
Humana Press
Year
2009
ISBN
978-1-58829-980-2, 978-1-59745-409-4
DOI
10.1007/978-1-59745-409-4
ISSN
1064-3745
LCC
QP92 .G458 2009
Open Library ID
OL24378863M
Language
english
Format
PDF
Filesize
8 MB (8424244 bytes)
Series
Methods In Molecular Biology 506
Edition
1
Pages
490\500
Topic
Biology Genetics
Library
torrents.ru tech collections 2009-11-14
Time added
2010-01-11 02:35:34
Cell Biology; Stem Cells; Gene Therapy

Description

With the incredible potential of gene transfer into hematopoietic stem cells, active research in this field has become critically important. In Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols, leading scientists in the field provide a compendium of protocols which cover the subject comprehensively, from the purification and culture of various types of hematopoietic cells for subsequent genetic modification by vector development and technical issues of small and large scale vector production, to the complex issue of monitoring and biosafety studies related to gene-modified hematopoiesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which allow the experts to highlight tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols is an ideal, thorough resource to promote further research and the implementation of investigator-driven clinical studies using gene-modified hematopoietic cells.

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