ENGLISH

Transgenic Ascidians

By Yasunori Sasakura

Book information

Publisher
Springer Singapore
Year
2018
ISBN
978-981-10-7544-5, 978-981-10-7545-2
Language
english
Format
PDF
Filesize
10 MB (10363675 bytes)
Series
Advances in Experimental Medicine and Biology 1029
Edition
1st ed.
Pages
X, 201\201
Time added
2018-08-15 07:07:45
Life Sciences; Developmental Biology; Transgenics;

Description

This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research. Front Matter ....Pages i-x Introduction (Yasunori Sasakura)....Pages 1-3 Microinjection of Exogenous Nucleic Acids into Eggs: Ciona Species (Kenji Kobayashi, Yutaka Satou)....Pages 5-13 Practical Guide for Ascidian Microinjection: Phallusia mammillata (Hitoyoshi Yasuo, Alex McDougall)....Pages 15-24 Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi (Gaku Kumano)....Pages 25-35 Electroporation in Ascidians: History, Theory and Protocols (Robert W. Zeller)....Pages 37-48 The Use of cis-Regulatory DNAs as Molecular Tools (Kotaro Shimai, Takehiro G. Kusakabe)....Pages 49-68 Reporter Analyses Reveal Redundant Enhancers that Confer Robustness on Cis-Regulatory Mechanisms (Shigeki Fujiwara, Cristian Cañestro)....Pages 69-79 Investigating Evolutionarily Conserved Molecular Mechanisms Controlling Gene Expression in the Notochord (Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio)....Pages 81-99 Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos (Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen)....Pages 101-107 Germline Transgenesis in Ciona (Yasunori Sasakura)....Pages 109-119 The Enhancer Trap in Ciona (Yasunori Sasakura)....Pages 121-129 TALEN-Based Knockout System (Keita Yoshida, Nicholas Treen)....Pages 131-139 CRISPR Knockouts in Ciona Embryos (Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi)....Pages 141-152 Transgenic Techniques for Investigating Cell Biology During Development (Christina D. Cota)....Pages 153-164 Cellular Processes of Notochord Formation (William C. Smith)....Pages 165-177 Morphology and Physiology of the Ascidian Nervous Systems and the Effectors (Atsuo Nishino)....Pages 179-196 Back Matter ....Pages 197-201

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