ENGLISH

Protein Nanotechnology: Protocols, Instrumentation, and Applications

By Juliet A. Gerrard , Laura J. Domigan

Book information

Publisher
Springer US;Humana
Year
2020
ISBN
978-1-4939-9868-5, 978-1-4939-9869-2
Language
english
Format
PDF
Filesize
10 MB (10327783 bytes)
Series
Methods in Molecular Biology 2073
Edition
3rd ed. 2020
Pages
XIII, 331\330
Time added
2020-02-08 04:40:58
Life Sciences; Protein Science; Biotechnology; Nan

Description

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation, and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field. Front Matter ....Pages i-xiii Introduction to Protein Nanotechnology (Laura J. Domigan, Juliet A. Gerrard)....Pages 1-13 Front Matter ....Pages 15-15 Protein Expression in the Baculovirus-Insect Cell Expression System (Adetayo A. Adeniyi, Linda Hwee-Lin Lua)....Pages 17-37 Peroxiredoxin Proteins as Building Blocks for Nanotechnology (Frankie Conroy, N. Amy Yewdall)....Pages 39-54 Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins (Sarah R. Ball, Chi L. L. Pham, Victor Lo, Vanessa K. Morris, Ann H. Kwan, Margaret Sunde)....Pages 55-72 ATP Synthase: Expression, Purification, and Function (Meghna Sobti, Robert Ishmukhametov, Alastair G. Stewart)....Pages 73-84 Molecular Superglues: Discovery and Engineering Orthogonalization (Paul G. Young, Christopher J. Squire)....Pages 85-99 Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes (Samuel Lim, Douglas S. Clark, Dominic J. Glover)....Pages 101-115 Front Matter ....Pages 117-117 Adding Function to Protein Scaffolds (Kyle Webster, Luigi Sasso, Laura J. Domigan)....Pages 119-147 Virus-Derived Nanoparticles (Noor H. Dashti, Frank Sainsbury)....Pages 149-162 Strategies for Increasing Protein Stability (Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan et al.)....Pages 163-181 Generation of High-Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol (Francesco Canfarotta, Sergey A. Piletsky, Nicholas W. Turner)....Pages 183-194 Nanotechnology with S-layer Proteins (Bernhard Schuster, Uwe B. Sleytr)....Pages 195-218 Front Matter ....Pages 219-219 Preparation of Proteins and Macromolecular Assemblies for Cryo-electron Microscopy (Lou Brillault, Michael J. Landsberg)....Pages 221-246 Atomic Force Microscopy of Proteins (Yiran An, Sesha Sarathchandra Manuguri, Jenny Malmström)....Pages 247-285 Native Protein Mass Spectrometry (Timothy M. Allison, Mark T. Agasid)....Pages 287-299 Nanoparticle Tracking Analysis of β-Casein Nanocarriers (Hikmat Masri, Jared K. Raynes)....Pages 301-309 Molecular Dynamics Simulation of Proteins (Thomas A. Collier, Thomas J. Piggot, Jane R. Allison)....Pages 311-327 Back Matter ....Pages 329-331

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