Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities, and Biological Applications, Second Edition

Description

Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.Content: Chapter 1 On the Mechanism of Electrospray Ionization Mass Spectrometry (ESIMS) (pages 1–48): Paul Kebarle and Udo H. VerkerkChapter 2 Selectivity in Electrospray Ionization Mass Spectrometry (pages 49–73): Nadja B. Cech and Christie G. EnkeChapter 3 Electrochemistry of the Electrospray Ion Source (pages 75–122): Gary J. Van Berkel and Vilmos KerteszChapter 4 ESI Source Design (pages 123–146): Andries P. BruinsChapter 5 MALDI Ionization Mechanisms: An Overview (pages 147–183): Richard KnochenmussChapter 6 The Development of Matrix?Assisted Laser Desorption/Ionization Sources (pages 185–213): Peter B. O'ConnorChapter 7 A Comparison of MALDI Matrices (pages 215–244): Mahmud Hossain and Patrick A. LimbachChapter 8 MALDI Imaging Mass Spectrometry (pages 245–261): Alain Brunelle and Olivier LaprevoteChapter 9 Coupling ESI and MALDI Sources to the Quadrupole Mass Filter, Quadrupole Ion Trap, Linear Quadrupole Ion Trap, and Orbitrap Mass Analyzers (pages 263–344): Mark E. BierChapter 10 Time?of?Flight Mass Spectrometry (pages 345–364): Robert J. CotterChapter 11 Fourier Transform Ion Cyclotron Resonance and Magnetic Sector Analyzers for ESI and MALDI (pages 365–409): Jeremy J. Wolff and I. Jonathan AmsterChapter 12 Ion Mobility–Mass Spectrometry (pages 411–439): John A. McLean, J. Albert Schultz and Amina S. WoodsChapter 13 ESI, APCI, and MALDI a Comparison of the Central Analytical Figures of Merit: Sensitivity, Reproducibility, and Speed (pages 441–490): Thomas R. Covey, Bradley B. Schneider, Hassan Javaheri, J.C. Yves LeBlanc, Gordana Ivosev, Jay J. Corr and Peter KovarikChapter 14 Charge State Distributions in Electrospray and MALDI (pages 491–534): Yan Li and Richard B. ColeChapter 15 Probing Noncovalent Interactions by Electrospray Ionization and Matrix?Assisted Laser Desorption/Ionization (pages 535–570): Sonal Mathur, Alexis Nazabal and Renato ZenobiChapter 16 Ion Activation Methods for Tandem Mass Spectrometry (pages 571–630): Kristina Hakansson and John S. KlassenChapter 17 Dissociation of Even?Electron Ions (pages 631–682): Carlos Afonso, Richard B. Cole and Jean?Claude TabetChapter 18 The Role of Mass Spectrometry for Peptide, Protein, and Proteome Characterization (pages 683–721): A. Jimmy Ytterberg, Jason Dunsmore, Shirley H. Lomeli, Mario Thevis, Yongming Xie, Rachel R. Ogorzalek Loo and Joseph A. LooChapter 19 Carbohydrate Analysis by ESI and MALDI (pages 723–769): David J. HarveyChapter 20 Applications of ESI and MALDI to Lipid Analysis (pages 771–797): Xianlin HanChapter 21 In Vitro ADME Profiling and Pharmacokinetic Screening in Drug Discovery (pages 799–824): Daniel B. Kassel