Mitochondria, 2nd Edition

Description

This book provides an update on the step-by-step "how to" methods for the study mitochondrial structure, function, and biogenesis contained in the successful first edition. As in the previous edition, the biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls from each method. Content: Isolation and Subfractionation of Mitochondria from Animal Cells and Tissue Culture Lines Review Article Pages 3-44 Francesco Pallotti, Giorgio Lenaz Purification and Subfractionation of Mitochondria from the Yeast Saccharomyces cerevisiae Review Article Pages 45-64 Istvan R. Boldogh, Liza A. Pon Isolation and Subfractionation of Mitochondria from Plants Review Article Pages 65-90 A.H. Millar, A. Liddell, C.J. Leaver Biochemical Assays of Respiratory Chain Complex Activity Review Article Pages 93-119 Denise M. Kirby, David R. Thorburn, Douglass M. Turnbull, Robert W. Taylor Polarographic Assays of Respiratory Chain Complex Activity* Review Article Pages 121-133 Gaetano Villani, Giuseppe Attardi Optical Imaging Techniques (Histochemical, Immunohistochemical, and In Situ Hybridization Staining Methods) to Visualize Mitochondria Review Article Pages 135-154 Kurenai Tanji, Eduardo Bonilla Assay of Mitochondrial ATP Synthesis in Animal Cells and Tissues Review Article Pages 155-171 Cristofol Vives‐Bauza, Lichuan Yang, Giovanni Manfredi Measurement of the Ratio of Lactate to Pyruvate in Skin Fibroblast Cultures Review Article Pages 173-178 Nevi Mackay, Brian H. Robinson Assays of Fatty Acid β‐Oxidation Activity Review Article Pages 179-197 Michael J. Bennett Biochemical Assays for Mitochondrial Activity: Assays of TCA Cycle Enzymes and PDHc Review Article Pages 199-222 Ann Saada Reisch, Orly Elpeleg Assays of Cardiolipin Levels Review Article Pages 223-240 Michael Schlame Measurement of VDAC Permeability in Intact Mitochondria and in Reconstituted Systems Review Article Pages 241-260 Marco Colombini Methods for Studying Iron Metabolism in Yeast Mitochondria Review Article Pages 261-280 Sabine Molik, Roland Lill, Ulrich Mühlenhoff Imaging of Mitochondrial Polarization and Depolarization with Cationic Fluorophores Review Article Pages 283-295 John J. Lemasters, Venkat K. Ramshesh Biosensors for the Detection of Calcium and pH Review Article Pages 297-325 Paolo Pinton, Alessandro Rimessi, Anna Romagnoli, Andrea Prandini, Rosario Rizzuto Measurement of Membrane Permeability and the Permeability Transition of Mitochondria Review Article Pages 327-340 Naoufal Zamzami, Carine Maisse, Didier Métivier, Guido Kroemer Luciferase Expression for ATP Imaging: Application to Cardiac Myocytes Review Article Pages 341-352 Christopher J. Bell, Giovanni Manfredi, Elinor J. Griffiths, Guy A. Rutter Measurement of Reactive Oxygen Species in Cells and Mitochondria Review Article Pages 355-377 J.S. Armstrong, M. Whiteman Measurements of the Antioxidant Enzyme Activities of Superoxide Dismutase, Catalase, and Glutathione Peroxidase Review Article Pages 379-393 Cristofol Vives‐Bauza, Anatoly Starkov, Elena Garcia‐Arumi Methods for Measuring the Regulation of Respiration by Nitric Oxide Review Article Pages 395-416 Sruti Shiva, Paul S. Brookes, Victor M. Darley‐Usmar Methods for Determining the Modification of Protein Thiols by Reactive Lipids Review Article Pages 417-434 JooYeun Oh, Michelle S. Johnson, Aimee Landar Detection of Mutations in mtDNA Review Article Pages 437-463 Ali Naini, Sara Shanske Diagnostic Assays for Defects in mtDNA Replication and Transcription in Yeast and Humans Review Article Pages 465-479 Gerald S. Shadel, Bonnie L. Seidel‐Rogol Microdissection and Analytical PCR for the Investigation of mtDNA Lesions Review Article Pages 481-501 Sion L. Williams, Carlos T. Moraes Transmitochondrial Technology in Animal Cells Review Article Pages 503-524 Sandra R. Bacman, Carlos T. Moraes Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii Mitochondria Review Article Pages 525-548 Nathalie Bonnefoy, Claire Remacle, Thomas D. Fox Generation of Transmitochondrial Mice: Development of Xenomitochondrial Mice to Model Neurodegenerative Diseases Review Article Pages 549-569 Carl A. Pinkert, Ian A. Trounce In Vivo and In Organello Analyses of Mitochondrial Translation Review Article Pages 571-588 P. Fernández‐Silva, R. Acín‐Pérez, E. Fernández‐Vizarra, A. Pérez‐Martos, J.A. Enriquez Visualization of Mitochondria in Budding Yeast Review Article Pages 591-626 Theresa C. Swayne, Anna C. Gay, Liza A. Pon Visualization and Quantification of Mitochondrial Dynamics in Living Animal Cells Review Article Pages 627-682 Kurt J. De Vos, Michael P. Sheetz Cell‐Free Assays for Mitochondria–Cytoskeleton Interactions Review Article Pages 683-706 Istvan R. Boldogh, Liza A. Pon, Michael P. Sheetz, Kurt J. De Vos In Vitro Assays for Mitochondrial Fusion and Division Review Article Pages 707-720 Elena Ingerman, Shelly Meeusen, Rachel DeVay, Jodi Nunnari Electrophoretic Methods to Isolate Protein Complexes from Mitochondria Review Article Pages 723-741 Ilka Wittig, Hermann Schägger Analysis of Protein–Protein Interactions in Mitochondria Review Article Pages 743-759 Johannes M. Herrmann, Benedikt Westermann Analysis and Prediction of Mitochondrial Targeting Signals Review Article Pages 761-781 Shukry J. Habib, Walter Neupert, Doron Rapaport Import of Proteins into Mitochondria Review Article Pages 783-806 Diana Stojanovski, Nikolaus Pfanner, Nils Wiedemann Series Editors Page II Contents Pages V-XV Contributors to Volume 80 Pages XVII-XXI Preface Pages XXIII-XXIV Liza A. Pon, Eric A. Schon Appendix 1. Basic Properties of Mitochondria Review Article Pages 809-812 Luis J. García‐Rodríguez Appendix 2. Direct and Indirect Inhibitors of Mitochondrial ATP Synthesis Review Article Pages 813-826 Nanette Orme‐Johnson Appendix 3. Linearized Maps of Circular Mitochondrial Genomes from Representative Organisms Review Article Pages 827-829 Eric A. Schon Appendix 4. Mitochondrial Genetic Codes in Various Organisms Review Article Pages 831-833 Compiled by Eric A. Schon Appendix 5. Gene Products Present in Mitochondria of Yeast and Animal Cells Review Article Pages 835-876 Eric A. Schon Appendix 6. Changes in the Mitochondrial Transcriptome and Proteome Under Various Stresses and Growth Conditions Review Article Pages 877-887 Eric A. Schon Index Pages 889-911 Volumes in Series Pages 913-920