ENGLISH

Laboratory Manual for Microbiology Fundamentals: A Clinical Approach

By Steven Obenauf , Susan Finazzo

Book information

Publisher
McGraw Hill
Year
2021
ISBN
1260786099, 9781260786095
Language
english
Format
PDF
Filesize
108 MB (113108015 bytes)
Edition
4
Pages
381\384
Time added
2023-01-01 16:51:00

Description

This laboratory manual for allied health or general microbiology has been written with the student in mind. The authors have used their years of teaching microbiology and microbiology laboratory at all levels to identify and relate the fundamental concepts that are important to the understanding of the science and students’ success in their future field. They have included case studies to exemplify the relevance of the science and extensive visual imagery to help students understand and learn the content. Most importantly, the authors hope this manual will help students experience the thrill of bench science and share some of the enthusiasm they have for microbiology, a field of science that is dynamic, exciting and touches every aspect of your life. Cover Title Copyright About the Authors Contents Laboratory Overview: Introduction Laboratory Safety, Equipment, and Materials Tools of the Laboratory: Basic Techniques 1 Use of the Microscope 2 Aseptic Techniques 3 Isolation Streak Plate Eukaryotic Cells and Microorganisms 4 Microbial Phototrophs: Algae and Cyanobacteria 5 Protozoa 6 Fungi 7 Bacterial Motility: Hanging Drop Method Methods for Microscopic Analysis: Staining 8 Simple Staining and Smear Preparation 9 Negative Stain 10 Capsule Stain 11 Gram Stain 12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods) 13 Endospore Stain (Schaeffer-Fulton Method) Methods for Culturing and Diagnosing Microbes: Media 14 Blood Agar 15 Mannitol Salt Agar 16 Eosin Methylene Blue Agar 17 MacConkey Agar Microbial Nutrition, Ecology, and Growth 18 Nutrients and Growth: Chemical Content of Media 19 Osmotic Pressure and Growth 20 Oxygen and Growth: Aerotolerance 21 Oxygen and Growth: Catalase 22 Oxygen and Growth: Oxidase Food and Water Safety 23 Plate Count Treatment and Control of Microbial Growth 24 Killing by Ultraviolet Light 25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method) Microbial Genetics and Genetic Engineering 26 ELISA and Using Nucleotide BLAST (Dry Lab) 27 Analysis of DNA: Electrophoresis 28 Gene Transfer: Transformation Infectious Diseases 29 Parasitic Protozoa 30 Parasitic Worms 31 Transmission: Vectors of Disease 32 Transmission: Epidemiology Activity Diagnosing Infections 33 Gel Immunoprecipitation (Immunodiffusion) 34 Passive (Indirect) Agglutination 35 Rapid Immunoassays 36 Hemagglutinin Inhibition Assay 37 EnteroPluri-Test Environmental Microbiology 38 Soil Microbiology 39 Nitrate Reduction Hydrolytic Enzymes and Disease Mechanisms 40 DNase Test 41 Gelatin Hydrolysis 42 Starch Hydrolysis 43 Casein Hydrolysis Biochemical Testing and Diagnosis of Disease 44 Phenol Red Sugar Broth 45 Triple-Sugar Iron Agar 46 Litmus Milk 47 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions 48 Urease Test Putting It All Together 49 "Known" Bacteria for Biochemical Testing 50 Identification of Unknown Bacteria Index A B C D E F G H I K L M N O P Q R S T U V W X Y Z

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