ENGLISH

Techniques for Work with Plant and Soil Nematodes

By Roland N. Perry (editor) , David Hunt (editor) , Sergei Subbotin (editor)

Book information

Publisher
CABI
Year
2020
ISBN
1786391759, 9781786391759
Language
english
Format
PDF
Filesize
21 MB (21588924 bytes)
Pages
376\313
Time added
2022-09-07 14:40:49

Description

Techniques for Work with Plant and Soil Nematodes is an up-to-date, comprehensive book covering the practicalities of working with and studying soil and plant nematodes. Written by an international team of experts, this book is highly illustrated and provides thorough coverage of methods while allowing for relevant information to be located quickly. It includes the fundamental traditional techniques and new methodologies, covering: sampling; extraction; estimating numbers; handling, fixing, staining, mounting; culturing techniques; figure preparation, measurement and image processing; electron microscopy techniques; behavioral and physiological assays; and cytogenetic, biochemical and molecular biology techniques. This book is an essential resource for anyone involved in plant nematology needing to refer to a readily available methodology standard, including students of nematology and parasitology, university lecturers and researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much needed compendium of the spectrum of information needed to work with these microscopic organisms. Contents About the Editors Contributors Preface 1 Sampling • Loes J.M.F. den Nijs, Jon Pickup and Ralf-Udo Ehlers 2 Methods for Nematode Extraction • Nicole Viaene, Johannes Hallmann and Leendert P.G. Molendijk 3 Estimating Numbers • Johannes Hallmann, Matthias Daub and Wim Wesemael 4 Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes • Wim Wesemael 5 Handling, Fixing, Staining and Mounting Nematodes • Jonathan D. Eisenback and David J. Hunt 6 Culturing Techniques • Rosa H. Manzanilla-López and Ralf-Udo Ehlers 7 Measuring Nematodes and Preparation of Figures • Weimin Ye and David J. Hunt 8 Electron Microscopy Techniques • Vladimir V. Yushin, Myriam Claeys, Jan L.M. Leunissen and Julia K. Zograf 9 Behavioural and Physiological Assays • Roland N. Perry 10 Staining Chromosomes • Jonathan D. Eisenback 11 Isoelectric Focusing of Proteins • Janete A. Brito 12 Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) • Sergei A. Subbotin 13 Isolation and Characterization of Tandem Repeats in Nematode Genomes • Philippe Castagnone-Sereno 14 Characterization of Nematode Mitochondrial Genomes • Danny A. Humphreys-Pereira, Taeho Kim and Joong-Ki Park 15 Phylogenetic Analysis of DNA Sequence Data • Sergei A. Subbotin Index

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