Protein Gel Detection and Imaging: Methods and Protocols

Description

This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field. Front Matter ....Pages i-xiii Protein Stains and Applications (Pazhani Sundaram)....Pages 1-14 The Roles of Acetic Acid and Methanol During Fixing and Staining Proteins in an SDS–Polyacrylamide Electrophoresis Gel (J. P. Dean Goldring)....Pages 15-18 Multicolored Prestained Standard Protein Marker Generation Using a Variety of Remazol Dyes for Easy Visualization of Protein Bands During SDS-PAGE (Gaurav Kumar)....Pages 19-25 Coomassie Brilliant Blue Staining of Polyacrylamide Gels (Claudia Arndt, Stefanie Koristka, Anja Feldmann, Ralf Bergmann, Michael Bachmann)....Pages 27-30 A Simple, Time-Saving Dye Staining of Proteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Using Coomassie Blue (Wei-hua Dong, Fang Wang, Jun-he Zhang, Yan-sheng Zhou, Ling-ye Zhang, Tian-yun Wang)....Pages 31-35 Application of Heat to Quickly Stain and Destain Proteins Stained with Coomassie Blue (Biji T. Kurien, R. Hal Scofield)....Pages 37-45 Silver Staining Techniques of Polyacrylamide Gels (Nicole Berndt, Ralf Bergmann, Claudia Arndt, Stefanie Koristka, Michael Bachmann)....Pages 47-52 Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry (Sun-Young Hwang, Jung-Kap Choi)....Pages 53-64 Detection of Phosphoproteins in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Using 8-Quinolinol Stain (Sun-Young Hwang, Xu Wang, Jung-Kap Choi)....Pages 65-73 Microwave-Assisted Protein Staining, Destaining, and In-Gel/In-Solution Digestion of Proteins (Jennie R. Lill, Victor J. Nesatyy)....Pages 75-86 Fluorescent Staining of Gels (Engelbert Buxbaum)....Pages 87-94 A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis (Jayanta K. Pal, Sunil K. Berwal, Rupali N. Soni)....Pages 95-103 TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE (Na Na, Jin Ouyang)....Pages 105-114 Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain (Padmaja Mehta-D’souza)....Pages 115-119 Curcumin/Turmeric as an Environment-Friendly Protein Gel Stain (Biji T. Kurien, Yaser Dorri, R. Hal Scofield)....Pages 121-131 Detection of Multiple Enzymes in Fermentation Broth Using Single PAGE Analysis (K. Divakar, J. Deepa Arul Priya, G. Panneer Selvam, M. Suryia Prabha, Ashwin Kannan, G. Nandhini Devi et al.)....Pages 133-138 Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis (Han-Min Chen)....Pages 139-147 A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins (Jelena Bogdanović Pristov, Ivan Spasojević)....Pages 149-158 Ten Minute Stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black (J. P. Dean Goldring, Robert G. E. Krause)....Pages 159-163 In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates (Isamu Kameshita, Noriyuki Sueyoshi, Atsuhiko Ishida)....Pages 165-172 Detection of Proteins in Polyacrylamide Gels via Prelabeling by Isatoic Anhydride (Kazem Asadollahi, Saharnaz Rafiee, Gholamhossein Riazi)....Pages 173-177 Fluorescent Protein Visualization Immediately After Gel Electrophoresis Using an In-Gel Trichloroethanol Photoreaction with Tryptophan (Carol L. Ladner-Keay, Raymond J. Turner, Robert A. Edwards)....Pages 179-190 Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel (Surbhi Desai, Boguslawa R. Dworecki, Marie C. Nlend)....Pages 191-205 Zymographic Determination of Intrinsic Specific Activity of Oxidases in the Presence of Interfering Proteins (Tien Canh Le, Mircea Alexandru Mateescu, Samaneh Ahmadifar, Lucia Marcocci, Paola Pietrangeli)....Pages 207-221 A Simple Method for Detecting Phosphorylation of Proteins by Using Zn2+-Phos-Tag SDS-PAGE at Neutral pH (Gaurav Kumar)....Pages 223-229 Principle and Method of Silver Staining of Proteins Separated by Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (Gaurav Kumar)....Pages 231-236 Heat/Pressure Treatment with Detergents Significantly Increases Curcumin Solubility and Stability: Its Use as an Environment-Friendly Protein Gel Stain (Biji T. Kurien, Rohit Thomas, Adam Payne, R. Hal Scofield)....Pages 237-246 Fungal Laccase Efficiently Destains Coomassie Brilliant Blue-R-250 Stained Polyacrylamide Gels (Gaurav Kumar)....Pages 247-253 Destaining Coomassie Brilliant Blue-Stained Sodium Dodecyl Sulfate–Polyacrylamide Protein Gels Using a Household Detergent (Rachna Aggarwal, Biji T. Kurien)....Pages 255-258 Paper Adsorbents Remove Coomassie Blue from Gel Destain and Used Gel Stain in an Environment-Friendly Manner (Yaser Dorri, Biji T. Kurien)....Pages 259-268 Gel Drying Methods (Anja Feldmann, Nicole Berndt, Ralf Bergmann, Michael Bachmann)....Pages 269-271 Stained Gels Can Be Stored for Several Months in Nonsealed Polyethylene Bags (Biji T. Kurien, R. Hal Scofield)....Pages 273-279 Radiolabeling and Analysis of Labeled Proteins (Nicole Berndt, Ralf Bergmann, Michael Bachmann)....Pages 281-285 Back Matter ....Pages 287-289