ENGLISH

Chromosome and Genomic Engineering in Plants: Methods and Protocols

Book information

Publisher
Humana Press
Year
2016
ISBN
1493949292, 9781493949298
DOI
10.1007/978-1-4939-4931-1
ISSN
1064-3745
Language
english
Format
PDF
Filesize
9 MB (9418793 bytes)
Series
Methods in Molecular Biology 1469
Pages
xii+218\231
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2016-08-25 20:57:04

Description

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.

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