Biophysical Osteoblast Stimulation for Bone Grafting and Regeneration: From Basic Science to Clinical Applications
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This book integrates the current basic and clinical knowledge in the area of biophysical stimulation of osteoblast for in vitro and in vivo live bone generation. This innovative methodology and its applications are presented and discussed in several clinical conditions: treatment of fracture nonunion, spine stabilization, bone replacement after tumor resections, stabilization of failed joint endoprostheses in revision surgery and in treatment of bone loss (osteoporosis and osteopenia). The author analyses different types of biophysical stimulation of osteoblasts for bone regeneration, e.g. mechanical (static and alternating, including distraction osteogenesis), electromagnetic (pulsed, alternating, static), light (at different spectrum range, including laser), acoustic (including ultrasound), RF etc. Moreover, he summarizes and discusses the most significant findings for in vitro bone generation and its resulting clinical use as autologous bone graft without surgical morbidity. Reflecting the author’s extensive experience this book is an excellent source of knowledge and a valuable aid to clinical practice for all orthopedic surgeons, fellows, and researchers wanting to gain insights into this promising field. Preface Historic Insights Contents About the Author Part I: In Vitro Bone Generation by Biophysical Stimulation. Prospective for Autologous Bone Grafting 1: The Theoretical Context of Biophysical Stimulation of Osteoblasts References 2: Selected Research Methodologies of Biophysical Stimulation of Osteoblast 2.1 Osteoblast Explant Cultures 2.2 End Parameters 2.3 Cell Death and Proliferation Estimation 2.4 Cellular Maturation Estimation 2.5 Cell Cycle Profile 2.5.1 The Assessment of Glycolytic Activity 2.5.2 Experimental Setups for External Application of Alternating Biophysical Energy 2.6 Visible Light Irradiance 2.7 Electromagnetic Field 2.8 Mechanical Stimulation 2.9 The Strengths and Weaknesses of the Methodologies References 3: Determination of In Vitro Generated Bone Tissue References 4: The Osseointegration Potential of Engineered Bone-Like Tissue μm 4.1 The Bone-Like Tissue for In Vivo Implantation References 5: The Clinical Potential of the In Vitro Generated Bone-Like Tissue 5.1 Translation from Basic Research to Clinical Practice 5.2 The Perspective for the Clinical Use of in Vitro-Generated Bone-Like Material 5.3 Conclusion References Part II: Clinical Applications of Biophysical Stimulation for Bone Regeneration 6: Local Vibration for Fracture Healing References 7: Whole-Body Vibration Enhances Bone Regeneration: For Treatment of Osteoporosis References 8: Distraction Osteogenesis References 9: Evolving Clinical Modalities for Bone Regeneration by Biophysical Stimulation 9.1 Electromagnetic Stimulation for Bone Fracture Healing 9.2 Fracture Healing by Ultrasound Stimulation 9.3 Fracture Healing by Radiofrequency (RF) Electromagnetic Fields References 10: To Summarize
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