Skeletal muscle development

Description

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>. Read more... Abstract: This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Read more... Front Matter ....Pages i-xii Front Matter ....Pages 1-1 Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells (Kohei Miyata, Masashi Naito, Tomoko Miyata, Sho Mokuda, Hiroshi Asahara)....Pages 3-13 Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells (Karl Kamhei So, Xianlu Laura Peng, Hao Sun, Huating Wang)....Pages 15-25 Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle (Tamar E. Sztal, Peter D. Currie, Robert J. Bryson-Richardson)....Pages 27-35 Front Matter ....Pages 37-37 Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS (Jordan Blondelle, Jean-Paul Pais de Barros, Fanny Pilot-Storck, Laurent Tiret)....Pages 39-60 Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells (C. Hai Ly, James G. Ryall)....Pages 61-73 Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures (Victor M. Aguilar, Benjamin D. Cosgrove)....Pages 75-92 Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle (Robert N. Judson, Marcela Low, Christine Eisner, Fabio M. Rossi)....Pages 93-103 Human Satellite Cell Isolation and Xenotransplantation (Steven M. Garcia, Stanley Tamaki, Xiaoti Xu, Jason H. Pomerantz)....Pages 105-123 Front Matter ....Pages 125-125 Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro (Manami Kodaka, Xiaoyin Xu, Zeyu Yang, Junichi Maruyama, Yutaka Hata)....Pages 127-134 Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells (Toshifumi Asano, Toru Ishizuka, Hiromu Yawo)....Pages 135-145 Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes (Gio C. Suh, Archana Bettadapur, Jeffrey W. Santoso, Megan L. McCain)....Pages 147-163 Front Matter ....Pages 165-165 Quantification of Embryonic Myofiber Development by Immunofluorescence (Harika Nagandla, M. David Stewart)....Pages 167-176 How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation (Maximilian Michael Saller, Paolo Alberton, Andrea B. Huber, Rosa-Eva Huettl)....Pages 177-192 Front Matter ....Pages 193-193 Membrane Repair Assay for Human Skeletal Muscle Cells (Romain Carmeille, Coralie Croissant, Flora Bouvet, Anthony Bouter)....Pages 195-207 Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle (Richard J. Mills, Holly K. Voges, Enzo R. Porrello, James E. Hudson)....Pages 209-224 Back Matter ....Pages 225-226