ENGLISH

Gene Essentiality: Methods and Protocols

By Long Jason Lu (eds.)

Book information

Publisher
Humana Press
Year
2015
ISBN
1493923978, 9781493923977
DOI
10.1007/978-1-4939-2398-4
ISSN
1064-3745
LCC
2014959295
Language
english
Format
PDF
Filesize
6 MB (6476252 bytes)
Series
Methods in Molecular Biology 1279
Edition
1
Pages
248\261
Topic
Biology Genetics
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-02-03 15:44:47
Human Genetics; Microbial Genetics and Genomics

Description

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

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