Biomaterials for Tissue Engineering

Description

This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study. Front Matter ....Pages i-xiv Engineering Citric Acid-Based Porous Scaffolds for Bone Regeneration (Jacqueline J. Masehi-Lano, Eun Ji Chung)....Pages 1-10 Multifunctional Self-Assembling Peptide-Based Nanostructures for Targeted Intracellular Delivery: Design, Physicochemical Characterization, and Biological Assessment (Yejiao Shi, Ran Lin, Honggang Cui, Helena S. Azevedo)....Pages 11-26 Electrospinning Functionalized Polymers for Use as Tissue Engineering Scaffolds (Lesley W. Chow)....Pages 27-39 Low-Temperature Deposition Modeling of β-TCP Scaffolds with Controlled Bimodal Porosity (E. Papastavrou, P. Breedon, D. Fairhurst)....Pages 41-54 Three-Dimensional Hydrogel-Based Culture to Study the Effects of Toxicants on Ovarian Follicles (Hong Zhou, Ariella Shikanov)....Pages 55-72 Layer-by-Layer Engineered Polymer Capsules for Therapeutic Delivery (Rona Chandrawati)....Pages 73-84 Controlling Fibrin Network Morphology, Polymerization, and Degradation Dynamics in Fibrin Gels for Promoting Tissue Repair (Erin P. Sproul, Riley T. Hannan, Ashley C. Brown)....Pages 85-99 Biofunctionalization of Poly(acrylamide) Gels (Julieta I. Paez, Aleeza Farrukh, Oya Ustahüseyin, Aránzazu del Campo)....Pages 101-114 Synthetic PEG Hydrogel for Engineering the Environment of Ovarian Follicles (Uziel Mendez, Hong Zhou, Ariella Shikanov)....Pages 115-128 Engineering Human Neural Tissue by 3D Bioprinting (Qi Gu, Eva Tomaskovic-Crook, Gordon G. Wallace, Jeremy M. Crook)....Pages 129-138 High-Throughput Formation of Mesenchymal Stem Cell Spheroids and Entrapment in Alginate Hydrogels (Charlotte E. Vorwald, Steve S. Ho, Jacklyn Whitehead, J. Kent Leach)....Pages 139-149 Crimped Electrospun Fibers for Tissue Engineering (Pen-hsiu Grace Chao)....Pages 151-159 In Vitro Model of Macrophage-Biomaterial Interactions (Claire E. Witherel, Pamela L. Graney, Kara L. Spiller)....Pages 161-176 Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide Synthesis (E. Thomas Pashuck)....Pages 177-192 H2S Delivery from Aromatic Peptide Amphiphile Hydrogels (Kuljeet Kaur, Yun Qian, John B. Matson)....Pages 193-208 Back Matter ....Pages 209-212