Preclinical MRI: Methods and Protocols

Description

This volume discusses a variety of preclinical MRI methods and protocols to help technicians and researchers conduct studies in their respective fields. This book is organized into 7 parts: Part 1 covers the basics of MRI physics, relaxation, image contrast, and main acquisition sequences; Part 2 describes methodologies for diffusion, perfusion, and functional imaging; Part 3 looks at in vivo spectroscopy; Part 4 explores special MRI techniques that are less known in the field; Parts 5 and 6 discuss MRIs and MRSs in animal models of disease and the applications used to study them, and Part 7 looks at anesthesia and advanced contrast agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Preclinical MRI: Methods and Protocols is a valuable resource for researchers interested in expanding their knowledge in this developing field. Front Matter ....Pages i-xiv Front Matter ....Pages 1-1 Introduction to MRI Physics (Gary V. Martinez)....Pages 3-19 Basic Pulse Sequences in Magnetic Resonance Imaging (Daniel Calle, Teresa Navarro)....Pages 21-37 Front Matter ....Pages 39-39 Dynamic Susceptibility Contrast MRI in Small Animals (Pilar López-Larrubia)....Pages 41-57 Preclinical Arterial Spin Labeling Measurement of Cerebral Blood Flow (Eric R. Muir)....Pages 59-70 Dynamic Contrast-Enhanced MRI (Jennifer Moroz, Stefan A. Reinsberg)....Pages 71-87 Diffusion-Weighted Magnetic Resonance Imaging (Irene Guadilla, Daniel Calle, Pilar López-Larrubia)....Pages 89-101 Diffusion Tensor Imaging (DTI) (Silvia Lope-Piedrafita)....Pages 103-116 Mapping Functional Connectivity in the Rodent Brain Using Electric-Stimulation fMRI (Laura Pérez-Cervera, José María Caramés, Luis Miguel Fernández-Mollá, Andrea Moreno, Begoña Fernández, Elena Pérez-Montoyo et al.)....Pages 117-134 Functional Diffusion Magnetic Resonance Imaging (Rita Maria Rocha Oliveira, Irene Guadilla, Pilar López-Larrubia)....Pages 135-147 Front Matter ....Pages 149-149 In Vivo 1H Magnetic Resonance Spectroscopy (M. Carmen Muñoz-Hernández, María Luisa García-Martín)....Pages 151-167 In Vivo Heteronuclear Magnetic Resonance Spectroscopy (Blanca Lizarbe, Antoine Cherix, Rolf Gruetter)....Pages 169-187 1H Spectroscopic Imaging of the Rodent Brain (Rui V. Simões, Emma Muñoz-Moreno, Raúl Tudela, Guadalupe Soria)....Pages 189-202 Front Matter ....Pages 203-203 Susceptibility Weighted MRI in Rodents at 9.4 T (Ferdinand Schweser, Marilena Preda, Robert Zivadinov)....Pages 205-234 Biomedical 19F MRI Using Perfluorocarbons (Tuba Güden-Silber, Sebastian Temme, Christoph Jacoby, Ulrich Flögel)....Pages 235-257 Rodent Abdominal Adipose Tissue Imaging by MR (Bhanu Prakash KN, Jadegoud Yaligar, Sanjay K. Verma, Venkatesh Gopalan, S. Sendhil Velan)....Pages 259-268 Cardiac MRI in Small Animals (Min-Chi Ku, Till Huelnhagen, Thoralf Niendorf, Andreas Pohlmann)....Pages 269-284 In Utero MRI of Mouse Embryos (Jiangyang Zhang, Dan Wu, Daniel H. Turnbull)....Pages 285-296 Oxygenation Imaging by Nuclear Magnetic Resonance Methods (Heling Zhou, Nuria Arias-Ramos, Pilar López-Larrubia, Ralph P. Mason, Sebastián Cerdán, Jesús Pacheco-Torres)....Pages 297-313 Molecular Magnetic Resonance Imaging (mMRI) (Maxime Gauberti, Antoine P. Fournier, Denis Vivien, Sara Martinez de Lizarrondo)....Pages 315-327 Front Matter ....Pages 329-329 Magnetic Resonance Spectroscopy Studies of Mouse Models of Cancer (Menglin Cheng, Kristine Glunde)....Pages 331-345 MRI in the Study of Animal Models of Neurodegenerative Diseases (Nyoman D. Kurniawan)....Pages 347-375 MRI in the Study of Animal Models of Stroke (Pedro Ramos-Cabrer, Daniel Padro)....Pages 377-392 Front Matter ....Pages 393-393 Assessment of Blood Brain Barrier Leakage with Gadolinium-Enhanced MRI (Min-Chi Ku, Sonia Waiczies, Thoralf Niendorf, Andreas Pohlmann)....Pages 395-408 In Vivo Pharmacokinetics of Magnetic Nanoparticles (Carlos Caro, M. Carmen Muñoz-Hernández, Manuel Pernia Leal, María Luisa García-Martín)....Pages 409-419 Front Matter ....Pages 421-421 Anesthesia and Monitoring of Animals During MRI Studies (Jordi L. Tremoleda, Sven Macholl, Jane K. Sosabowski)....Pages 423-439 Advanced Contrast Agents for Multimodal Biomedical Imaging Based on Nanotechnology (Daniel Calle, Paloma Ballesteros, Sebastián Cerdán)....Pages 441-457 Back Matter ....Pages 459-464