ENGLISH

Microcharacterrization of Proteins, Second Edition

Book information

Publisher
Wiley-VCH Verlag GmbH
Year
1999
ISBN
9783527300846, 9783527613960
DOI
10.1002/9783527613960
Language
english
Format
PDF
Filesize
5 MB (5751581 bytes)
Pages
333\333
Orientation
yes
Scanned
yes
Time added
2013-08-01 04:00:00

Description

Proteomics - the analysis of the whole set of proteins and their functions in a cell - is based on the revolutionary developments which have been achieved in protein analysis during the last years. The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships. The second edition of the successful title Microcharacterization of Proteins presents a current overview of modern protein analysis: From sample preparation to sequence analysis, mass spectrometry and bioinformatics it informs about the tools needed in protein research. This makes the book indispensable for everyone involved in proteomics! Content: Chapter I.1 Microcharacterization of Proteins (pages 2–10): Friedrich Lottspeich and Roland KellnerChapter II.1 Microseparation Techniques I: High Resolution Gel?Electrophoretic Techniques: Qualitative, Quantitative and Micropreparative Applications (pages 13–24): Angelika Gorg and Reiner WestermeierChapter II.2 Microseparation Techniques II: Gel Electrophoresis for Sample Preparation in Protein Chemistry (pages 25–34): Hermann SchaggerChapter II.3 Microseparation Techniques III: Electroblotting (pages 35–48): Christoph EckerskornChapter II.4 Microseparation Techniques IV: Analysis of Peptides and Proteins by Capillary Electrophoresis (pages 49–65): Christine SchwerChapter II.5 Microseparation Techniques V: High Performance Liquid Chromatography (pages 67–85): Maria Serwe, Martin Bluggel and Helmut E. MeyerChapter II.6 Sample Preparation I: Removal of Salts and Detergents (pages 87–95): Dorian Immler, Ehrenfried Mehl, Friedrich Lottspeich and Helmut E. MeyerChapter II.7 Sample Preparation II: Chemical and Enzymatic Fragmentation of Proteins (pages 97–116): Roland Kellner and Tony HouthaeveChapter III.1 Amino Acid Analysis (pages 119–139): Roland Kellner, Helmut E. Meyer and Friedrich LottspeichChapter III.3 Chemical Methods for Protein Sequence Analysis (pages 141–158): Friedrich Lottspeich, Tony Houthaeve and Roland KellnerChapter III.3 Analyzing Post?Translational Protein Modifications (pages 159–175): Helmut E. MeyerChapter III.4 Analysis of Biopolymers by Matrix?Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (pages 177–196): Ute Bahr, Michael Karas and Franz HillenkampChapter III.5 MALDI Postsource Decay Mass Analysis (pages 197–211): Bernhard Spengler, Pierre Chaurand and Peter BoldChapter III.6 Electrospray Mass Spectrometry (pages 213–234): Jorg W. Metzger, Christoph Eckerskorn, Christoph Kempter and Beate BehnkeChapter III.7 Fourier?Transform Ion Cyclotron Resonance Mass Spectrometry (FT?ICR?MS) (pages 235–244): Jorg W. MetzgerChapter III.8 Sequence Analysis of Proteins and Peptides by Mass Spectrometry (pages 245–273): Christoph Siethoff, Christiane Lohaus and Helmut E. MeyerChapter IV.1 Internet Resources for Protein Identification and Characterization (pages 277–299): Pierre?Alain Binz, Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Ron D. Appel and Denis F. HochstrasserChapter IV.2 Protein Sequences and Genome Databases (pages 301–317): Hans?Werner Mewes, Andreas Maierl and Dimitrij Frishman

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