ENGLISH

Cytokine Protocols (Methods in Molecular Biology Vol 249)

By Marc De Ley

Book information

Publisher
Humana Press
Year
2003
ISBN
9780896039483, 9781592596676, 089603948X
LCC
QR185.8.C95 C957 2004
Google Books ID
BUWMMnptGfQC
Open Library ID
OL17617134M
Language
english
Format
PDF
Filesize
2 MB (2166018 bytes)
Edition
1
Pages
239\239
Topic
Biology Molecular
Orientation
yes
Scanned
no
Time added
2011-06-04 13:46:07

Description

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

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