ENGLISH

Plant Gene Silencing: Methods and Protocols

By Kirankumar S. Mysore , Muthappa Senthil-Kumar (eds.)

Book information

Publisher
Humana Press
Year
2015
ISBN
1493924524, 9781493924523
DOI
10.1007/978-1-4939-2453-0
ISSN
1064-3745
Google Books ID
2015930614
Language
english
Format
PDF
Filesize
8 MB (8286296 bytes)
Series
Methods in Molecular Biology 1287
Edition
1
Pages
307\321
Topic
Biology Genetics
Orientation
yes
Paginated
yes
Scanned
portrait
Time added
2015-03-08 13:51:35
Plant Genetics & Genomics

Description

This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.

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