ENGLISH

Microinjection: Methods and Protocols

By Chengyu Liu; Yubin Du

Book information

Publisher
Humana Press
Year
2018
ISBN
1493988301, 9781493988303
Language
english
Format
PDF
Filesize
18 MB (18500005 bytes)
Edition
Hardcover
Pages
540\544
Time added
2018-11-24 12:50:40

Description

This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations. Front Matter ....Pages i-xiv Microinjection and Micromanipulation: A Historical Perspective (Wenhao Xu)....Pages 1-16 Production of Transgenic Mice by Pronuclear Microinjection (Xin-an Pu, Anthony P. Young, H. Michael Kubisch)....Pages 17-41 Transgene Recombineering in Bacterial Artificial Chromosomes (Michael G. Zeidler, Thomas L. Saunders)....Pages 43-69 Using TARGATT™ Technology to Generate Site-Specific Transgenic Mice (Ruby Yanru Chen-Tsai)....Pages 71-86 Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method (Takuya Sato, Takehiko Ogawa)....Pages 87-98 Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer (Yubin Du, Wen Xie, Fan Zhang, Chengyu Liu)....Pages 99-114 Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection (Pinghu Liu, Yan Li, Jingqi Lei, Lijin Dong)....Pages 115-137 Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice (Yubin Du, Wen Xie, Fan Zhang, Uimook Choi, Chengyu Liu, Colin L. Sweeney)....Pages 139-168 Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique (Melissa A. Scott, Yueh-Chiang Hu)....Pages 169-178 Delivery of CRISPR-Cas9 into Mouse Zygotes by Electroporation (Wenning Qin, Haoyi Wang)....Pages 179-190 Generation of Conditional Knockout Mice by Sequential Insertion of Two loxP Sites In Cis Using CRISPR/Cas9 and Single-Stranded DNA Oligonucleotides (Ye Liu, Yubin Du, Wen Xie, Fan Zhang, Douglas Forrest, Chengyu Liu)....Pages 191-210 Improvement of Mouse Cloning from Any Type of Cell by Nuclear Injection (Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama)....Pages 211-228 The CARD Method for Simple Vitrification of Mouse Oocytes: Advantages and Applications (Naomi Nakagata, Jorge Sztein, Toru Takeo)....Pages 229-242 The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm (Toru Takeo, Jorge Sztein, Naomi Nakagata)....Pages 243-256 Isolation and Analysis of a Genome-Edited Single-Hepatocyte from a Cas9 Transgenic Mouse Line (Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B. Gurumurthy, Masahiro Sato, Takayuki Shindo)....Pages 257-271 Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology (Tuija M. Alcantar, Xin Y. Rairdan)....Pages 273-294 Molecular Aspects of Zinc Finger Nucleases (ZFNs)-Mediated Gene Editing in Rat Embryos (Xiaoxia Cui)....Pages 295-312 Organ Generation from Knockedout Rat Blastocysts Complemented with Pluripotent Stem Cells (Masumi Hirabayashi, Shinichi Hochi)....Pages 313-326 Generation of Rabbit Models by Gene Editing Nucleases (Dongshan Yang, Jie Xu, Y. Eugene Chen)....Pages 327-345 Production of Genetically Engineered Porcine Embryos by Handmade Cloning (Rong Li, Jinxin Miao, Zhongde Wang)....Pages 347-360 Electrofusion of 2-Cell Embryos for Porcine Tetraploid Embryo Production (Qingran Kong, Zhonghua Liu)....Pages 361-371 Gene Knockouts in Goats Using CRISPR/Cas9 System and Somatic Cell Nuclear Transfer (Zhiqiang Fan, Min Yang, Misha Regouski, Irina A. Polejaeva)....Pages 373-390 Generating Goat Mammary Gland Bioreactors for Producing Recombinant Proteins by Gene Targeting (Liyou An, Lan Yang, Yuejin Huang, Yong Cheng, Fuliang Du)....Pages 391-401 Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes (Ellen J. Collarini, Philip A. Leighton, Marie-Cecile Van de Lavoir)....Pages 403-430 Using Microinjection to Generate Genetically Modified Caenorhabditis elegans by CRISPR/Cas9 Editing (Jyoti Iyer, Nicole DeVaul, Tyler Hansen, Ben Nebenfuehr)....Pages 431-457 Microinjection in Zebrafish for Genome Editing and Functional Studies (Wuhong Pei, Shawn M. Burgess)....Pages 459-474 Microinjection of Marine Fish Eggs (Rie Goto, Taiju Saito, Takahiro Matsubara, Etsuro Yamaha)....Pages 475-487 Generating Gene Knockout Oryzias latipes and Rice Field Eel Using TALENs Method (Daji Luo, Ke Feng, Zuoyan Zhu, Wei Hu)....Pages 489-506 Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus (Yuki Shibata, Lingyu Bao, Liezhen Fu, Bingyin Shi, Yun-Bo Shi)....Pages 507-524 Microinjection of Live Mammalian Cells: A Delivery Method that Provides Added Versatility to the Study of Cellular Function (Robert F. Gahl)....Pages 525-536 Back Matter ....Pages 537-540

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