Genome editing in neurosciences

Description

This book is open access under a CC BY 4.0 license. CRISPR-Cas9 is a rapid, efficient, versatile and relatively cheap method for dissecting the molecular pathways that are the basis of life, as well as for investigating and potentially rectifying faults in these pathways that result in disease. This book reviews how CRISPR-Cas9 and other genome editing techniques are advancing our understanding of development and function in the nervous system, uncovering the molecular causes of neurological disorders and providing tools for gene therapy. Front Matter ....Pages i-xi In Vitro Modeling of Complex Neurological Diseases (Frank Soldner, Rudolf Jaenisch)....Pages 1-19 Aquatic Model Organisms in Neurosciences: The Genome-Editing Revolution (Jean-Stéphane Joly)....Pages 21-29 Genome-Wide Genetic Screening in the Mammalian CNS (Mary H. Wertz, Myriam Heiman)....Pages 31-39 CRISPR/Cas9-Mediated Knockin and Knockout in Zebrafish (Shahad Albadri, Flavia De Santis, Vincenzo Di Donato, Filippo Del Bene)....Pages 41-49 Dissecting the Role of Synaptic Proteins with CRISPR (Salvatore Incontro, Cedric S. Asensio, Roger A. Nicoll)....Pages 51-62 Recurrently Breaking Genes in Neural Progenitors: Potential Roles of DNA Breaks in Neuronal Function, Degeneration and Cancer (Frederick W. Alt, Pei-Chi Wei, Bjoern Schwer)....Pages 63-72 Neuroscience Research Using Non-human Primate Models and Genome Editing (Noriyuki Kishi, Hideyuki Okano)....Pages 73-81 Multiscale Genome Engineering: Genome-Wide Screens and Targeted Approaches (Neville E. Sanjana)....Pages 83-86 Using Genome Engineering to Understand Huntington’s Disease (Barbara Bailus, Ningzhe Zhang, Lisa M. Ellerby)....Pages 87-101 Therapeutic Gene Editing in Muscles and Muscle Stem Cells (Mohammadsharif Tabebordbar, Jason Cheng, Amy J. Wagers)....Pages 103-123