ENGLISH

Pichia Protocols

By David R. Higgins (ed.) , James M. Cregg (ed.)

Book information

Publisher
Humana Press
Year
1998
ISBN
978-0-89603-421-1
DOI
10.1385/0896034216
Language
english
Format
PDF
Filesize
19 MB (19792072 bytes)
Series
Methods in Molecular Biology 103
Pages
262\262
Topic
Biology
Library
mexmat
Time added
2009-07-20 03:45:11
Cell Biology

Description

David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods-developed by world-class researchers in their own laboratories-contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

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