Microbial Lipid Production: Methods and Protocols

Description

The volume explores methods for isolation and characterization of lipids, and their production using various oleaginous organisms. The protocols presented in the book consider sugars derived from different substrates including chemically pre-treated agricultural residues, industrial residues containing lignin, food wastes, and industrial waste water in an approachable format. The book also discusses applications that use oleaginous organisms to transform substrates into a variety of products including bio-crude, high value fatty acids, biofuels such as biodiesel, neutral lipids, volatile fatty acids, and surfactants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Microbial Lipid Production: Methods and Protocols is a valuable resource aimed at novice researchers who are working on improving their knowledge and skills in this developing field. Front Matter ....Pages i-xiv Microbial Lipid Alternatives to Plant Lipids (A. Daniel Jones, Kyria L. Boundy-Mills, G. Florin Barla, Sandeep Kumar, Bryan Ubanwa, Venkatesh Balan)....Pages 1-32 Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts (Irnayuli R. Sitepu, Antonio L. Garay, Tomas Cajka , Oliver Fiehn, Kyria L. Boundy-Mills)....Pages 33-50 Fungi (Mold)-Based Lipid Production (Yan Yang, Fatemeh Heidari, Bo Hu)....Pages 51-89 Qualitative and Quantitative Estimation of Bacterial Lipid Production (Gangatharan Muralitharan, Manickam Gayathri, Sumathy Shunmugam)....Pages 91-101 Rhodococcus and Yarrowia-Based Lipid Production Using Lignin-Containing Industrial Residues (Rosemary K. Le, Kristina M. Mahan, Arthur J. Ragauskas)....Pages 103-120 Quantification of Lipid Content in Oleaginous Biomass Using Thermogravimetry (Balakrishna Maddi, Agasteswar Vadlamani, Sridhar Viamajala, Sasidhar Varanasi)....Pages 121-129 Extraction and Characterization of Lipids from Macroalgae (David R. Nobles Jr., Schonna R. Manning)....Pages 131-140 Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbes (Xiao-Ling Tang, Ya-Ping Xue)....Pages 141-150 Extraction Methods Used to Separate Lipids from Microbes (Balakrishna Maddi)....Pages 151-159 Novel Microbial Modification Tools to Convert Lipids into Other Value-Added Products (Priya Kumari, Farnaz Yusuf, Naseem A. Gaur)....Pages 161-171 Alkaline and Alkaline-Oxidative Pretreatment and Hydrolysis of Herbaceous Biomass for Growth of Oleaginous Microbes (Jacob D. Crowe, Muyang Li, Daniel L. Williams, Alex D. Smith, Tongjun Liu, David B. Hodge)....Pages 173-182 Laboratory Conversion of Cultivated Oleaginous Organisms into Biocrude for Biofuel Applications (Eboibi Blessing, Umakanta Jena, Senthil Chinnasamy)....Pages 183-193 Life Cycle Analysis of Producing Microbial Lipids and Biodiesel: Comparison with Plant Lipids (Tom Bradley, Daniel Maga)....Pages 195-214 Assessment of Fuel Quality Parameters and Selection of Bacteria Using PROMETHEE–GAIA Algorithm (Sumathy Shunmugam, Manickam Gayathri, Gangatharan Muralitharan)....Pages 215-227 Production of High-Value Polyunsaturated Fatty Acids Using Microbial Cultures (Mingjie Jin, Rui Zhai, Zhaoxian Xu, Zhiqiang Wen)....Pages 229-248 Screening for Oily Yeasts Able to Convert Hydrolysates from Biomass to Biofuels While Maintaining Industrial Process Relevance (Patricia J. Slininger, Bruce S. Dien, Joshua C. Quarterman, Stephanie R. Thompson, Cletus P. Kurtzman)....Pages 249-283 Conversion of Microbial Lipids to Biodiesel and Basic Lab Tests for Analysis of Fuel-Quality Parameters (Annaliese K. Franz, Cody Yothers)....Pages 285-310 Impact of Culture Conditions on Neutral Lipid Production by Oleaginous Yeast (Irene Fakankun, Maryam Mirzaei, David B. Levin)....Pages 311-325 Producing Oleaginous Microorganisms Using Wastewater: Methods and Guidelines for Lab- and Industrial-Scale Production (Kayla M. Rude, Tyler J. Barzee, Annaliese K. Franz)....Pages 327-355 Volatile Fatty Acid Production from Anaerobic Digestion of Organic Residues (Sibel Uludag-Demirer, Wei Liao, Goksel N. Demirer)....Pages 357-367 Producing Oleaginous Organisms Using Food Waste: Challenges and Outcomes (Singaram Jayanthi, Arun Kumar Thalla)....Pages 369-381 Microbial Surfactants: Alternative to Vegetable Oil Surfactants (Eduardo J. Gudiña, Lígia R. Rodrigues)....Pages 383-393 Evaluation of Bacterial Lipid Production: Quantitative and Qualitative Measurements: Tips and Guidelines (Sima Modiri, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi)....Pages 395-403 Production of Oleaginous Organisms or Lipids Using Sewage Water and Industrial Wastewater (Farha Deeba, Vikas Pruthi, Yuvraj S. Negi)....Pages 405-418 Back Matter ....Pages 419-422